RelA/NFkB p65 [p Ser276] Inhibitor Peptide Set

NF-kB has been shown to regulate the expression of a number of genes whose products are involved in inflammation, viral replication, carcinogenesis, antiapoptosis, invasion, and metastasis. Specific adhesion molecules, chemokines, inflammatory cytokines, and cell cycle regulatory genes are affected. Thus, agents that can suppress NF-kB activation have the potential to be treatments for inflammatory diseases and cancer.

The Ser276 site of p65 is phosphorylated during NF-kB activation, allowing p65 nuclear translocation. This p65 inhibitory peptide contains a Ser276 site that is phosphorylated during NF-kB activation, thereby blocking p65 Ser276 phosphorylation (Bharti et al, 2003; Takada et al, 2004). The NF-kB p65 (Ser276), also known as PTD-p65-P1, inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable (Derossi et al, 1994). The control peptide consists of only the PTD sequence. The PTD-p65-P1 peptide can inhibit binding of recombinant p65 protein to the DNA in a dose dependent manner and maximum inhibition occurs at 50 uM (Figure 1). It can also inhibit TNF- induced NF-kB activation in vivo. PTD-p65-P1 suppresses TNF-induced NF-kB activation by 25% at 100 uM and completely at 150 uM (figure 2).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: B/N, Forced Spectroscopy, Func, In vitro, WB.

Showing Publications 1 - 10 of 23. Show All 23 Publications. Collapse Publications.

Publications using NBP2-26505	Applications	Species
Yu J, Berga SL, Zou W et al. IL-1B Inhibits Connexin 43 and Disrupts Decidualization of Human Endometrial Stromal Cells through ERK1/2 and p38 MAP Kinase Endocrinology 2017-09-11 [PMID: 28938400] (B/N, Human)	B/N	Human
Chen Jy, Lai Ys, Tsai Hj et al. The oncometabolite R-2-hydroxyglutarate activates NF-kB-dependent tumor-promoting stromal niche for acute myeloid leukemia cells. Sci Rep 2016-08-31 [PMID: 27577048]
Basson MD, Zeng B, Downey C et al. Increased extracellular pressure stimulates tumor proliferation by a mechanosensitive calcium channel and PKC-b. Molecular Oncology. 2014-10-23 [PMID: 25454347] (In vitro, Func, B/N)	In vitro, Func, B/N
Hutcherson Ja, Bagaitkar J, Nagano K et al. Porphyromonas gingivalis RagB is a pro-inflammatory STAT4 agonist. Mol Oral Microbiol. 2014-11-21 [PMID: 25418117]
Lowe JM, Menendez D, Bushel PR et al. p53 and NF-kB Coregulate Proinflammatory Gene Responses in Human Macrophages. Cancer Res. 2014-04-15 [PMID: 24737129] (Forced Spectroscopy, Human) Details: Primary macrophages, Fig S2B.	Forced Spectroscopy	Human
DLC1 suppresses NF-kappaB activity in prostate cancer cells due to its stabilizing effect on adherens junctions. Tripathi V, Popescu NC, Zimonjic DB Springerplus [PMID: 24683532] (Func, Human)	Func	Human
Qin S, Taglienti M, Cai L et al. c-Met and NF-kB-dependent overexpression of Wnt7a and -7b and Pax2 promotes cystogenesis in polycystic kidney disease. J Am Soc Nephrol. 2012-08-01 [PMID: 22677559] Details: Products cited: 1. IKK-gamma NEMO Binding Domain Inhibitory Peptide (IMG-2000/IMG-2000-5): Fig 6E (immortalized mouse kidney epithelial cell line) and Fig 6F (mouse kidney explants). The cell line and explants were treated with IMG-2000. Readout assay: RT
Bhooshan Neha, Giger Maryellen, Lan Li et al. Combined use of T2-weighted MRI and T1-weighted dynamic contrast-enhanced MRI in the automated analysis of breast lesions. Magn Reson Med. 2011-08-01 [PMID: 21523818] (Human)		Human
Wang H, Brown J, Gu Z et al. Convergence of the mammalian target of rapamycin complex 1- and glycogen synthase kinase 3-B-signaling pathways regulates the innate inflammatory response. J Immunol. 2011-05-01 [PMID: 21422248] (WB, Human) Details: WB: Human PBMC's were pretreated with the NF-kB p65 inhibitory peptide then treated with Rapamycin in the presence or absence of LPS, Fig 5B.	WB	Human
Majewski Pawel M, Thurston Robert D, Ramalingam Rajalakshmy et al. Cooperative role of NF-{kappa}B and poly(ADP-ribose) polymerase 1 (PARP-1) in the TNF-induced inhibition of PHEX expression in osteoblasts. J Biol Chem. 2010-11-05 [PMID: 20817730]
Buggins AG, Pepper C, Patten PE et al. Interaction with vascular endothelium enhances survival in primary chronic lymphocytic leukemia cells via NF-kappaB activation and de novo gene transcription. Cancer Res. 2010-10-01 [PMID: 20736369] Details: p65 inhibitory peptide (IMG-2001). CLL (chronic lymphocytic leukemia) cells were incubated with p65 inhibitory peptide and cultured in the presence or absence of endothelial cells, Fig 5.
Balasubramani Anand, Shibata Yoichiro, Crawford Gregory E et al. Modular utilization of distal cis-regulatory elements controls Ifng gene expression in T cells activated by distinct stimuli. Immunity. 2010-07-23 [PMID: 20643337] (Mouse)		Mouse
Mohamed JS, Lopez MA, Cox GA, Boriek AM. Anisotropic regulation of Ankrd2 gene expression in skeletal muscle by mechanical stretch. FASEB J. 2010-09-01 [PMID: 20442316] Details: 1. NF-kB p50 (NLS) Inhibitory Peptide Set (IMG-2004): Fig 4AB.
Chastre E, Abdessamad M, Kruglov A et al. TRIP6, a novel molecular partner of the MAGI-1 scaffolding molecule, promotes invasiveness. FASEB J. 2009-03-01 [PMID: 19017743] Details: HeLa and HEk293 cell lysates were transiently transfected with Flag labeled MAG-1b.
Poylin V, Fareed MU, O'Neal P et al. The NF-kappaB inhibitor curcumin blocks sepsis-induced muscle proteolysis. Mediators Inflamm. 2008-01-01 [PMID: 18389075] Details: Incubated muscles from septic rats, muscles were incubated in the presence of 100 um inhibitory peptide or 100 um control peptide. Treatment of the muscles with the inhibitory peptide for 2 hr resulted in an ~30% reduction of protein breakdown compared to
Katz J, Zhang P, Martin M et al. Toll-like receptor 2 is required for inflammatory responses to Francisella tularensis LVS. Infect Immun. 2006-05-01 [PMID: 16622218] (Mouse) Details: IMG-2001: (mouse dentritic cells), Figs. 5, 6.		Mouse
Ichikawa H, Aggarwal BB. Guggulsterone inhibits osteoclastogenesis induced by receptor activator of nuclear factor-kappaB ligand and by tumor cells by suppressing nuclear factor-kappaB activation. Clin Cancer Res. 2006-01-15 [PMID: 16428513]
Fan W, Agarwal N, Cooper NG. The role of CaMKII in BDNF-mediated neuroprotection of retinal ganglion cells (RGC-5). Brain Res. 2006-01-05 [PMID: 16337157]
Zou JY, Crews FT. TNF alpha potentiates glutamate neurotoxicity by inhibiting glutamate uptake in organotypic brain slice cultures: neuroprotection by NF kappa B inhibition. Brain Res. 2005-02-09 [PMID: 15713255]
Takada Y, Singh S, Aggarwal BB. Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis. J Biol Chem. 2004-04-09 [PMID: 14711835]
Bharti AC, Donato N, Aggarwal BB. Curcumin (diferuloylmethane) inhibits constitutive and IL-6-inducible STAT3 phosphorylation in human multiple myeloma cells. J Immunol. 2003-10-01 [PMID: 14500688]
Skowron J. [The effect of iron on peritoneal macrophage activity and sperm phagocytosis in rats]. Ann Acad Med Stetin. 2000-01-01 [PMID: 11712318]
Derossi D, Joliot AH, Chassaing G, Prochiantz A. The third helix of the Antennapedia homeodomain translocates through biological membranes. J Biol Chem. 1994-04-08 [PMID: 8144628]
Show All 23 Publications. Collapse Publications.

Array NBP2-26505

• RelA/NFkB p65 Antibodies
• RelA/NFkB p65 Antibody Pack
• RelA/NFkB p65 ELISA Kits
• RelA/NFkB p65 Inhibitors
• RelA/NFkB p65 Kit
• RelA/NFkB p65 Lysate
• RelA/NFkB p65 Proteins