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RelA/NFkB p65 Antibody (532301) [Phycoerythrin]

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HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse RelA/NF kappa B p65 PE-conjugated Monoclonal Antibody (Catalog # IC5078P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse RelA/NF kappa B p65 PE-conjugated Monoclonal Antibody (Catalog # IC5078P, filled histogram) or isotype control antibody (Catalog ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
532301
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC5078U-100UG
Alexa Fluor 405
IC5078V-100UG
Alexa Fluor 488
IC5078G
Alexa Fluor 532
FAB5078X
Alexa Fluor 594
IC5078T-100UG
Alexa Fluor 647
IC5078R-100UG
Alexa Fluor 700
IC5078N-100UG
Alexa Fluor 750
IC5078S-100UG
Allophycocyanin
IC5078A
Biotin
FAB5078B
CoraFluor 1
FAB5078CL1
DyLight 350
FAB5078D
DyLight 405
FAB5078E
DyLight 488
FAB5078K
DyLight 550
FAB5078L
DyLight 594
FAB5078M
DyLight 650
FAB5078W
DyLight 680
FAB5078Y
DyLight 755
FAB5078Z
FITC
FAB5078F
HRP
FAB5078H
Janelia Fluor 525
FAB5078JF525
Janelia Fluor 549
FAB5078I
Janelia Fluor 635
FAB5078JF635
Janelia Fluor 646
FAB5078J
Janelia Fluor 669
FAB5078JF669
PerCP
FAB5078C
Unconjugated
MAB5078
mFluor Violet 450 SE
FAB5078MFV450
mFluor Violet 500 SE
FAB5078MFV500
mFluor Violet 610 SE
FAB5078MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB5078
Carrier Free
MAB5078
Carrier Free
IC5078A
Carrier Free
IC5078G
Carrier Free
MAB5078-SP
Carrier Free
IC5078V-100UG
Carrier Free
IC5078U-100UG
Carrier Free
IC5078T-100UG
Carrier Free
IC5078S-100UG
Carrier Free
IC5078R-100UG
Carrier Free
IC5078N-100UG
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RelA/NFkB p65 Antibody (532301) [Phycoerythrin] Summary

Immunogen
E. coli-derived recombinant human RelA/NF kappa B p65 isoform 1
Asn456-Ser551
Accession # Q04206
Specificity
Detects human and mouse RelA/NF kappa B p65 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RELA
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC5078P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RelA/NFkB p65 Antibody (532301) [Phycoerythrin]

  • MGC131774
  • nf kb p65
  • NFkB p65
  • NF-kB p65
  • NFKB3
  • NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
  • p65
  • p65RelA
  • rela p65
  • RelA
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

RelA p65 (v-rel reticuloendotheliosis viral oncogene homolog A) is a 65 kDa member of the NF kappa B family of nuclear transcription factors. Dimers of p65 with the p50 subunit are the most common form of the NF kappa B transcription factor, but dimers with it or other family members can also occur. Upon activation, RelA p65 forms an heterotetramer and moves into the nucleus where it binds to specific DNA sequences. An alternatively spliced isoform that lacks amino acids (aa) 222‑231 (p65 delta ) does not bind DNA. Over the sequence used as an immunogen, human RelA p65 shares 96% and 98% aa identity with mouse and rat RelA p65, respectively. This portion includes one of eight potential ser/thr phosphorylation sites, two acetylation sites, and most of the Rel homology domain that interacts with I kappa B inhibitors.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RelA/NFkB p65 Antibody (IC5078P)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(3)
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Mouse
(3)
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Showing Publications 1 - 5 of 5.
Publications using IC5078P Applications Species
P Shafiei-Ja, DG Helou, BP Hurrell, E Howard, C Quach, JD Painter, L Galle-Treg, M Li, YE Loh, O Akbari CD200-CD200R immune checkpoint engagement regulates ILC2 effector function and ameliorates lung inflammation in asthma Nature Communications, 2021-05-05;12(1):2526. 2021-05-05 [PMID: 33953190] (Flow Cytometry, Mouse) Flow Cytometry Mouse
P Shafiei-Ja, BP Hurrell, L Galle-Treg, DG Helou, E Howard, J Painter, R Lo, G Lewis, P Soroosh, O Akbari DR3 stimulation of adipose resident ILC2s ameliorates type 2 diabetes mellitus Nat Commun, 2020-09-18;11(1):4718. 9/18/2020 [PMID: 32948777]
BP Hurrell, L Galle-Treg, PS Jahani, E Howard, DG Helou, H Banie, P Soroosh, O Akbari TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity Cell Rep, 2019-12-24;29(13):4509-4524.e5. 2019-12-24 [PMID: 31875557] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Galle-Treger L, Hurrell BP, Lewis G Autophagy is critical for group 2 innate lymphoid cell metabolic homeostasis and effector function J Allergy Clin Immunol. NULL [PMID: 31738991]
L Galle-Treg, I Sankaranar, BP Hurrell, E Howard, R Lo, H Maazi, G Lewis, H Banie, AL Epstein, P Hu, VK Rehan, FD Gilliland, H Allayee, P Soroosh, AH Sharpe, O Akbari Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes Nat Commun, 2019-02-12;10(1):713. 2019-02-12 [PMID: 30755607] (Flow Cytometry, Mouse) Flow Cytometry Mouse

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Secondary Antibodies

 

Isotype Controls

Additional RelA/NFkB p65 Products

Blogs on RelA/NFkB p65.

Check out the latest blog posts on RelA/NFkB p65.
Killing two birds with one stone: Treating inflammation and cancer by inhibiting prolyl-4-hydroxylase-1
By Jamshed Arslan Pharm.D. The cell’s oxygen-sensing machinery comprises prolyl-4-hydroxylases (P4Hs 1-3, PHDs 1-3, or EGLN 1-3) and their canonical target hypoxia-inducible factors (HIFs). When oxygen levels ...  Read full blog post.
The effect of antioxidants and the NFkB p65 pathway in inflammation
NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit...  Read full blog post.
NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol RELA
Uniprot