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Recombinant Viral HCMV IL-10 Protein, CF

 (10 Publications)
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Product Details

Summary
Reactivity VSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
117-VL-025
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Recombinant Viral HCMV IL-10 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit LPS-induced IL‑1 beta secretion by human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.1-0.6 ng/mL.
Source
E. coli-derived viral IL-10 protein
Ala26-Lys176
Accession #
N-terminal Sequence
Ala26
Structure / Form
Noncovalently-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
CeHV15gBCRF1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
117-VL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Viral HCMV IL-10 Protein, CF

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen‑stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3).

  1. Moore, K.W. et al. (2001) Annu. Rev. Immunol. 19:683.
  2. Kotenko, S.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1695.
  3. Spencer, J.V. et al. (2002) J. Virol. 76:1285.

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Publications for IL-10 (117-VL/CF)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Bioassay, ELISA (Standard), In Vivo, Western Blot.


Filter By Application
Bioassay
(5)
ELISA (Standard)
(3)
In Vivo
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using 117-VL/CF Applications Species
S Waters, S Lee, A Irish, P Price Challenging the Conventional Interpretation of HCMV Seronegativity Microorganisms, 2021-11-18;9(11):. 2021-11-18 [PMID: 34835508] (ELISA (Standard), Human) ELISA (Standard) Human
CA Valle Oseg, JV Spencer Human cytomegalovirus interleukin-10 enhances matrigel invasion of MDA-MB-231 breast cancer cells Cancer Cell Int, 2017-02-13;17(0):24. 2017-02-13 [PMID: 28228690] (Bioassay, Human) Bioassay Human
J Stuchl?, V Kanderov , M Vlkov , I He?manov , L Sl mov , O Pel k, E Taraldsrud, D J¡lek, P Kr l¡c Kov, B Fevang, M Trkov , O Hru? k, E Fro?kov , A ?ediv , J Litzman, T Kalina Common Variable Immunodeficiency patients with a phenotypic profile of immunosenescence present with thrombocytopenia Sci Rep, 2017-01-05;7(0):39710. 2017-01-05 [PMID: 28054583] (ELISA (Standard)) ELISA (Standard)
Bishop R, Valle Oseguera C, Spencer J Human Cytomegalovirus interleukin-10 promotes proliferation and migration of MCF-7 breast cancer cells. Cancer Cell Microenviron, 2015-01-01;2(1):. 2015-01-01 [PMID: 26023679] (Bioassay, Human) Bioassay Human
Valle Oseguera , Cendy A, Spencer , Juliet V cmvIL-10 stimulates the invasive potential of MDA-MB-231 breast cancer cells. PLoS ONE, 2014-02-10;9(2):e88708. 2014-02-10 [PMID: 24520416] (Bioassay, Human) Bioassay Human
Forster S, Brandt M, Mottok D, Zschuttig A, Zimmermann K, Blattner F, Gunzer F, Pohlmann C Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct. BMC Biotechnol, 2013-10-05;13(0):82. 2013-10-05 [PMID: 24093616] (Western Blot) Western Blot
Dziurzynski K, Wei J, Qiao W, Hatiboglu MA, Kong LY, Wu A, Wang Y, Cahill D, Levine N, Prabhu S, Rao G, Sawaya R, Heimberger AB Glioma-associated cytomegalovirus mediates subversion of the monocyte lineage to a tumor propagating phenotype. Clin. Cancer Res., 2011-04-13;17(14):4642-9. 2011-04-13 [PMID: 21490182] (ELISA (Standard)) ELISA (Standard)
Chang WL, Barry PA, Szubin R, Wang D, Baumgarth N Human cytomegalovirus suppresses type I interferon secretion by plasmacytoid dendritic cells through its interleukin 10 homolog. Virology, 2009-06-13;390(2):330-7. 2009-06-13 [PMID: 19524994] (Bioassay, Human) Bioassay Human
van Putten SM, Wubben M, Hennink WE, van Luyn MJ, Harmsen MC The downmodulation of the foreign body reaction by cytomegalovirus encoded interleukin-10. Biomaterials, 2008-11-26;30(5):730-5. 2008-11-26 [PMID: 19038442] (In Vivo, Mouse) In Vivo Mouse
Gruber SG, Gloria Luciani M, Grundtner P, Zdanov A, Gasche C Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1. Eur. J. Immunol., 2008-12-01;38(12):3365-75. 2008-12-01 [PMID: 19016528] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol CeHV15gBCRF1
Uniprot