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Recombinant Rat CCL2/JE/MCP-1 Protein

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price
Carrier Free
3144-JE-025/CF
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Recombinant Rat CCL2/JE/MCP-1 Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED50 for this effect is 30-150 ng/mL.
Source
Mouse myeloma cell line, NS0-derived rat CCL2/JE/MCP-1 protein
Gln24-Asn148
Accession #
N-terminal Sequence
No results obtained: Gln24 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
Ccl2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
25-35 kDa, reducing conditions
Publications
Read Publications using
3144-JE in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose and with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CCL2/JE/MCP-1 Protein

  • C-C motif chemokine ligand 2
  • CCL2
  • GDCF-2
  • HC11
  • HSMCR30
  • MCAF
  • Mcp1
  • MCP-1
  • SCYA2
  • SMC-CF

Background

Rat CCL2 is a member of the beta (C-C) subfamily of chemokines that is a chemoattractant for monocytes and basophils but not eosinophils or neutrophils (1-4). Rat CCL2 is secreted as a 14 kDa glycoprotein monomer (5) but noncovalent dimers probably occur (1). The first five amino acids of the mature protein are essential for activity; deletion of the N-terminal glutamine, which is pyrrolidone carboxylic acid-modified, dramatically decreases activity on basophils and, surprisingly, stimulates eosinophil chemotaxis (4). The rat CCL2 propeptide shares 82% amino acid (a.a.) identity with mouse CCL2 over the 148 a.a. sequence and 57%, 52%, 52%, 52% and 52% a.a. identity with equine, human, porcine, canine and guinea pig CCL2, respectively, over the first 100 aa. Rat and mouse CCL2 have a 49 aa extension at the C-terminus as compared to human CCL2. Fibroblasts, tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation. CCL2 is best known as a chemotactic agent for mononuclear cells. It also induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils, primarily due to interaction with CCR2 receptors on these cells. Additionally, it is believed to reduce IL-12 production by dendritic cells and promote a Th2 phenotype in CD4+ T cells. The role of CCL2 in recruiting monocytes to sites of inflammation is implicated in the pathology of multiple sclerosis, atherosclerosis and allergic asthma (6).

  1. VanCoillie, E.V. et al. (1999) Cytokine Growth Factor Rev. 10:61.
  2. Luther, S.A. and J.G. Cyster (2001) Nat. Immunol. 2:102.
  3. Lu, B. et al. (1998) J. Exp. Med. 187:601.
  4. Weber, M. et al. (1996) J. Exp. Med. 183:681.
  5. Yoshimura, T. et al. (1991) Biochem. Biophys. Res. Commun. 174:504.
  6. Daly, C. et al. (2003) Microcirculation 10:247.

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Publications for CCL2/JE/MCP-1 (3144-JE)(7)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(5)
In Vivo
(2)
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Rat
(7)
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Showing Publications 1 - 7 of 7.
Publications using 3144-JE Applications Species
H Tsushima, K Yamada Effects of adipokine administration to the hypothalamic preoptic area on body temperature in rats J. Pharmacol. Sci., 2020-07-10;0(0):. 2020-07-10 [PMID: 32684333] (In Vivo, Rat) In Vivo Rat
X Ding, W Yang, Q Ren, J Hu, S Yang, W Han, J Wang, X Wang, H Wang Serum IgG-induced microglial activation enhances neuronal cytolysis via the NO/sGC/PKG pathway in children with opsoclonus-myoclonus syndrome and neuroblastoma J Neuroinflammation, 2020-06-16;17(1):190. 2020-06-16 [PMID: 32546235] (Bioassay, Rat) Bioassay Rat
HT Lee, SP Liu, CH Lin, SW Lee, CY Hsu, HK Sytwu, CH Hsieh, WC Shyu A Crucial Role of CXCL14 for Promoting Regulatory T Cells Activation in Stroke Theranostics, 2017-02-08;7(4):855-875. 2017-02-08 [PMID: 28382159] (Bioassay, Rat) Bioassay Rat
Nathalie Strazielle, Rita Creidy, Christophe Malcus, José Boucraut, Jean-François Ghersi-Eg T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium. PLoS ONE, 2016-03-04;0(0):1932-6203. 2016-03-04 [PMID: 26942913] (Bioassay, Rat) Bioassay Rat
Huang C, Chen Y, Li A, Lu J, Wang H Minocycline, a microglial inhibitor, blocks spinal CCL2-induced heat hyperalgesia and augmentation of glutamatergic transmission in substantia gelatinosa neurons. J Neuroinflammation, 2014-01-10;11(0):7. 2014-01-10 [PMID: 24405660] (In Vivo, Rat) In Vivo Rat
Vomaske J, Denton M, Kreklywich C, Andoh T, Osborn J, Chen D, Messaoudi I, Orloff S, Streblow D Cytomegalovirus CC chemokine promotes immune cell migration. J Virol, 2012-08-22;86(21):11833-44. 2012-08-22 [PMID: 22915808] (Bioassay, Rat) Bioassay Rat
Bhangoo SK, Ren D, Miller RJ, Chan DM, Ripsch MS, Weiss C, McGinnis C, White FA CXCR4 chemokine receptor signaling mediates pain hypersensitivity in association with antiretroviral toxic neuropathy. Brain Behav. Immun., 2007-02-09;21(5):581-91. 2007-02-09 [PMID: 17292584] (Bioassay, Rat) Bioassay Rat

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Bioinformatics

Gene Symbol Ccl2
Uniprot