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Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF

 (9 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse VEGFR3/Flt-4 Fc Chimera (Catalog # 743-R3) at 5 μg/mL (100 μL/well) can bind Recombinant Mouse VEGF‑D (Catalog # 469-VD) with an apparent Kd <50 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse VEGFR3/Flt-4 protein
MetMouse VEGFR3
(Tyr25-Asp770)
Accession # P35917		IEGRDIDHuman IgG1
(Pro100-Lys330)
6-His tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Flt4
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
112 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
123 kDa with two additional minor bands at 73 kDa and 60 kDa, reducing conditions
Publications
Read Publications using
743-R3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

VEGFR3 (Flt-4), together with VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

In adults, VEGFR3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGFR3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGFR3 has been reported to serve as the receptors for VEGF-C and VEGF-D.

  1. Finnerty, H. et al. (1993) Oncogene 8:2293.
  2. Joukov, V. et al. (1996) EMBO J. 15:290.
  3. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA. 95:548.

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Recombinant Mouse
VEGFR3/Flt-4 Fc Chime ...
743-R3
Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF
Species: Mu
Applications: Binding Activity

Publications for VEGFR3/Flt-4 (743-R3)(9)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Antibody Absorption, Bioassay, In Vivo, Western Blot Control.


Filter By Application
Antibody Absorption
(1)
Bioassay
(1)
In Vivo
(6)
Western Blot Control
(1)
All Applications
Filter By Species
Mouse
(9)
All Species
Showing Publications 1 - 9 of 9.
Publications using 743-R3 Applications Species
I Martinez-C, Y Zhang, M Petkova, H Ortsäter, S Sjöberg, SD Castillo, P Brouillard, L Libbrecht, D Saur, M Graupera, K Alitalo, L Boon, M Vikkula, T Mäkinen Blockade of VEGF-C signaling inhibits lymphatic malformations driven by oncogenic PIK3CA mutation Nat Commun, 2020-06-08;11(1):2869. 2020-06-08 [PMID: 32513927] (Western Blot Control, Mouse) Western Blot Control Mouse
Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, Derecki N, Castle D, Mandell J, Lee K, Harris T, Kipnis J Structural and functional features of central nervous system lymphatic vessels. Nature, 2015-06-01;523(7560):337-41. 2015-06-01 [PMID: 26030524] (Antibody Absorption, Mouse) Antibody Absorption Mouse
Decio A, Taraboletti G, Patton V, Alzani R, Perego P, Fruscio R, Jurgensmeier J, Giavazzi R, Belotti D Vascular endothelial growth factor c promotes ovarian carcinoma progression through paracrine and autocrine mechanisms. Am J Pathol, 2014-02-06;184(4):1050-61. 2014-02-06 [PMID: 24508126] (In Vivo, Mouse) In Vivo Mouse
Conrady CD, Zheng M, Stone DU CD8+ T cells suppress viral replication in the cornea but contribute to VEGF-C-induced lymphatic vessel genesis. J. Immunol., 2012-05-30;189(1):425-32. 2012-05-30 [PMID: 22649204] (In Vivo, Mouse) In Vivo Mouse
Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling. Nature, 2012-03-18;484(7392):110-4. 2012-03-18 [PMID: 22426001] (Bioassay, Mouse) Bioassay Mouse
Wuest TR, Carr DJ VEGF-A expression by HSV-1-infected cells drives corneal lymphangiogenesis. J. Exp. Med., 2009-12-21;207(1):101-15, S1-2. 2009-12-21 [PMID: 20026662] (In Vivo, Mouse) In Vivo Mouse
Halin C, Tobler NE, Vigl B, Brown LF, Detmar M VEGF-A produced by chronically inflamed tissue induces lymphangiogenesis in draining lymph nodes. Blood, 2007-07-11;110(9):3158-67. 2007-07-11 [PMID: 17625067] (In Vivo, Mouse) In Vivo Mouse
Cursiefen C, Chen L, Saint-Geniez M, Hamrah P, Jin Y, Rashid S, Pytowski B, Persaud K, Wu Y, Streilein JW, Dana R Nonvascular VEGF receptor 3 expression by corneal epithelium maintains avascularity and vision. Proc. Natl. Acad. Sci. U.S.A., 2006-07-18;103(30):11405-10. 2006-07-18 [PMID: 16849433] (In Vivo, Mouse) In Vivo Mouse
Cao R, Bjorndahl MA, Gallego MI, Chen S, Religa P, Hansen AJ, Cao Y Hepatocyte growth factor is a lymphangiogenic factor with an indirect mechanism of action. Blood, 2006-01-19;107(9):3531-6. 2006-01-19 [PMID: 16424394] (In Vivo, Mouse) In Vivo Mouse

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Bioinformatics

Gene Symbol Flt4
Uniprot