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Recombinant Mouse VEGF 164 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
493-MV-005
Carrier Free
493-MV-025/CF
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Recombinant Mouse VEGF 164 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 0.8-4 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse VEGF protein
Ala27-Arg190
Accession #
N-terminal Sequence
Ala27
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Vegfa
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
19.4 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
42 kDa, non-reducing conditions
Publications
Read Publications using
493-MV/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 50 µg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse VEGF 164 Protein, CF

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF or VEGF‑A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by a cystine knot structure formed by eight conserved cysteine residues (4). Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164 and 188 aa isoforms found in mouse (2, 4). Isoforms other than VEGF120 and VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Mouse VEGF164 shares 97% aa sequence identity with corresponding regions of rat, 89% with human and porcine, 88% with bovine, and 90% with feline, equine and canine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). Human VEGF165 binds the semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2 (5). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (6, 7). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (8). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 9).

  1. Breier, G. et al. (1992) Development 114:521.
  2. Shima, D.T. et al. (1996) J. Biol. Chem. 271:3877.
  3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.
  4. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
  5. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049.
  6. Weis, S.M. and D.A. Cheresh (2005) Nature 437:497.
  7. Thurston, G. (2002) J. Anat. 200:575.
  8. Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246.
  9. Angelo, L.S. and R. Kurzrock (2007) Clin. Cancer Res. 13:2825.

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Publications for VEGF (493-MV/CF)(91)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, N/A, Porcine.

We have publications tested in 12 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Capture), ELISA (Control), ELISA (Standard), IP, Immunoprecipitation, In Vivo, Surface Plasmon Resonance, Western Blot, Western Blot Control.


Filter By Application
Binding Assay
(2)
Bioassay
(49)
Cell Culture
(3)
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(1)
ELISA (Control)
(1)
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IP
(1)
Immunoprecipitation
(1)
In Vivo
(31)
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All Applications
Filter By Species
Human
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Mouse
(81)
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N/A
(1)
Porcine
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All Species
Showing Publications 1 - 10 of 91. Show All 91 Publications.
Publications using 493-MV/CF Applications Species
L�tge, M;De Martin, A;Gil-Cruz, C;Perez-Shibayama, C;Stanossek, Y;Onder, L;Cheng, HW;Kurz, L;Cadosch, N;Soneson, C;Robinson, MD;Stoeckli, SJ;Ludewig, B;Pikor, NB; Conserved stromal-immune cell circuits secure B cell homeostasis and function Nature immunology 2023-05-18 [PMID: 37202489] (Bioassay, Mouse) Bioassay Mouse
V Buncha, KA Fopiano, L Lang, C Williams, A Horuzsko, JA Filosa, G Kapuku, Z Bagi Mice with endothelial cell-selective adhesion molecule deficiency develop coronary microvascular rarefaction and left ventricle diastolic dysfunction Physiological Reports, 2023-03-01;11(6):e15643. 2023-03-01 [PMID: 36946064] (Bioassay, Mouse) Bioassay Mouse
BN Sullivan, MA Baggett, C Guillory, M Jones, C Steele Neuroimmune Responses in a New Experimental Animal Model of Cerebral Aspergillosis MBio, 2022-08-30;0(0):e0225422. 2022-08-30 [PMID: 36040029] (In Vivo, Mouse) In Vivo Mouse
K Ma, G Singh, J Wang, I O-Sullivan, G Votta-Veli, B Bruce, AN Anbazhagan, AJ van Wijnen, HJ Im Targeting Vascular Endothelial Growth Factor Receptors as a Therapeutic Strategy for Osteoarthritis and Associated Pain International journal of biological sciences, 2023-01-01;19(2):675-690. 2023-01-01 [PMID: 36632459] (Bioassay, Mouse) Bioassay Mouse
Y Ichiyama, R Matsumoto, S Obata, O Sawada, Y Saishin, M Kakinoki, T Sawada, M Ohji Assessment of mouse VEGF neutralization by ranibizumab and aflibercept PLoS ONE, 2022-12-21;17(12):e0278951. 2022-12-21 [PMID: 36542626] (Western Blot, N/A) Western Blot N/A
S Yuan, KS Stewart, Y Yang, MD Abdusselam, SM Parigi, TY Feinberg, K Tumaneng, H Yang, JM Levorse, L Polak, D Ng, E Fuchs Ras drives malignancy through stem cell crosstalk with the microenvironment Nature, 2022-11-30;0(0):. 2022-11-30 [PMID: 36450983] (In Vivo, Mouse) In Vivo Mouse
D Pan, W Wu, G Zuo, X Xie, H Li, X Ren, C Kong, W Zhou, Z Zhang, M Waterfall, S Chen Sphingosine 1-phosphate receptor 2 promotes erythrocyte clearance by vascular smooth muscle cells in intraplaque hemorrhage through MFG-E8 production Cellular Signalling, 2022-07-26;98(0):110419. 2022-07-26 [PMID: 35905868] (Bioassay, Mouse) Bioassay Mouse
CY Tsai, JCC Wu, CJ Wu, SHH Chan Protective role of VEGF/VEGFR2 signaling against high fatality associated with hepatic encephalopathy via sustaining mitochondrial bioenergetics functions Oncogene, 2022-07-03;29(1):47. 2022-07-03 [PMID: 35786324] (Bioassay, Mouse) Bioassay Mouse
B Roy, Z Yang, G Pan, K Roth, M Agarwal, R Sharma, MC Petriello, SS Palaniyand Exposure to the Dioxin-like Pollutant PCB 126 Afflicts Coronary Endothelial Cells via Increasing 4-Hydroxy-2 Nonenal: A Role for Aldehyde Dehydrogenase 2 Oncogene, 2022-06-16;10(6):. 2022-06-16 [PMID: 35736936] (Bioassay, Mouse) Bioassay Mouse
Show All 91 Publications.

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FAQs for VEGF (493-MV/CF). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
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Bioinformatics

Gene Symbol Vegfa
Uniprot