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Recombinant Mouse MSP/MST1 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
6244-MS-025/CF
Carrier Free
6244-MS-025/CF
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Recombinant Mouse MSP/MST1 Protein Summary

Details of Functionality
Measured by its binding ability in a functional ELISA.

When Recombinant Mouse MSP R/Ron Fc Chimera (Catalog # 431-MS) is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse MSP/MST1 that produces 50% of the optimal binding response is found to be approximately 1 ‑ 6 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse MSP/MST1 protein
Met1-Glu716 (Cys677Ala)
Accession #
N-terminal Sequence
No results obtained for alpha ‑chain, sequencing might be blocked: Gln19 predicted ( alpha ‑chain) & Val489 ( beta ‑chain)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Mst1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
53.7 kDa ( alpha ‑chain) & 25 kDa ( beta ‑chain).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
61-62 kDa & 30-31 kDa, reducing conditions
Publications
Read Publications using
6244-MS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MSP/MST1 Protein

  • D3F15S2
  • EC 3.4.21
  • hepatocyte growth factor-like protein homolog
  • HGFL
  • HGFLhepatocyte growth factor-like protein
  • macrophage stimulating 1 (hepatocyte growth factor-like)
  • Macrophage stimulatory protein
  • Macrophage-stimulating protein
  • MSP
  • MSPDNF15S2
  • MST1
  • NF15S2
  • SF2

Background

Macrophage stimulating protein (MSP), also known as HGF‑like protein, and scatter factor‑2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro‑MSP) that contains a PAN/APPLE‑like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Mouse MSP shares 79% and 93% aa sequence identity with human and rat MSP, respectively and 42% aa sequence identity with mouse HGF. Pro‑MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF‑4 or RAR, respectively (3). Circulating pro‑MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45 ‑ 62 kDa alpha and a 25 ‑ 35 kDa beta chain (4, 5). Pro‑MSP can be activated by MT‑SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP, as well as the isolated beta chain, binds to MSP R/Ron with high‑affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS‑ or IFN‑induced iNOS and IL‑12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12). The substitution of cysteine 672 (in the beta chain) with alanine significantly increases the bioactivity of recombinant human MSP, apparently by limiting incorrect disulfide bond formation between the alpha and beta chains (13).
  1. Wang, M.-H. et al. (2002) Scand. J. Immunol.56:545.
  2. Degen, S.J. et al. (1991) Biochemistry 30:9781.
  3. Muraoka, R.S. et al. (1999) Endocrinology 140:187.
  4. Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720.
  5. Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573.
  6. Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771.
  7. Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999.
  8. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
  9. Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39.
  10. Kurihara, N. et al. (1998) Exp. Hematol. 26:1080.
  11. Morrison, A.C. et al. (2004) J. Immunol. 172:1825.
  12. Liu, Q.P. et al. (1999) J. Immunol. 163:6606.
  13. Wahl, R.C. et al. (1997) J. Biol. Chem. 272:15053.

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Recombinant Mouse
MSP/MST1 Protein
6244-MS
Recombinant Mouse MSP/MST1 Protein
Species: Mu
Applications: Bioactivity

Publications for MSP/MST1 (6244-MS)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol Mst1
Uniprot