Recombinant Mouse LIF Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Recombinant mouse LIF (leukemia inhibitory factor) is commonly used in cell culture to maintain the pluripotency of stem cells. LIF is a widely expressed pleiotropic member of the IL-6 family of cytokines (1-3). Mature mouse LIF is expressed as a highly and variably glycosylated 32-62 kDa monomer that shares 78%, 91%, 80%, 76%, and 78% aa sequence identity with human, rat, canine, bovine, and porcine LIF, respectively (4). LIF functions through a heterodimeric receptor complex containing a ligand-binding subunit, LIF R alpha /CD118, and a signal transducing subunit, gp130 (2, 4, 5). gp130 also serves as a subunit of the receptor complexes for Oncostatin M, Cardiotrophin-1, CNTF, IL-6, IL-11, and IL-27 (2, 5). A soluble form of mouse LIF R alpha can be generated by alternative splicing (6). Depending on the cells and their context, LIF either opposes or favors differentiation (2, 7). LIF produced by the uterine endometrium supports successful implantation of the embryo, promotes proliferation and maintenance of pluripotency in embryonic stem cells, and favors proliferation of progenitor cell types such as hematopoietic stem cells (2, 5, 7). However, excess LIF blocks differentiation of embryoid bodies, indicating the importance of LIF regulation (2, 5). LIF is produced by activated CD4⁺ T cells and is required by the thymic epithelium to support T cell maturation (2, 3). Its expression is upregulated by neuronal injury, and it promotes motor neuron survival and oligodendrocyte myelination (2, 3, 8). It is produced by the adrenal cortex and likely enhances adrenal production of cortisol and aldosterone (9). LIF can also function as an autocrine growth factor in some pancreatic cancers, but it induces differentiation in the myeloid leukemic cell line M1 (1, 10). Tumor cell-derived LIF can also induce formation of immunosuppressive tumor-associated macrophages (11). LIF promotes endometrial remodeling and differentiation of adipocytes and cardiac smooth muscle cells (2, 3, 12). It promotes regulatory T cell and inhibits Th17 cell differentiation, thus down-regulating inflammation and contributing to immune tolerance during pregnancy and in the nervous system (2, 3, 5, 7).

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using 8878-LF/CF	Applications	Species
Zhu, Z;Chen, X;Guo, A;Manzano, T;Walsh, PJ;Wills, KM;Halliburton, R;Radko-Juettner, S;Carter, RD;Partridge, JF;Green, DR;Zhang, J;Roberts, CWM; Mitotic bookmarking by SWI/SNF subunits Nature 2023-06-01 [PMID: 37225980] (Bioassay, Mouse)	Bioassay	Mouse
FA Graca, M Rai, LC Hunt, A Stephan, YD Wang, B Gordon, R Wang, G Quarato, B Xu, Y Fan, M Labelle, F Demontis The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy Nature Communications, 2022-05-02;13(1):2370. 2022-05-02 [PMID: 35501350] (Bioassay, Mouse)	Bioassay	Mouse
I Kelava, I Chiaradia, L Pellegrini, AT Kalinka, MA Lancaster Androgens increase excitatory neurogenic potential in human brain organoids Nature, 2022-01-19;0(0):. 2022-01-19 [PMID: 35046577] (Bioassay, Mouse)	Bioassay	Mouse
K Noh, H Lee, TY Choi, Y Joo, SJ Kim, H Kim, JY Kim, JW Jahng, S Lee, SY Choi, SJ Lee Negr1 controls adult hippocampal neurogenesis and affective behaviors Mol. Psychiatry, 2019-01-16;0(0):. 2019-01-16 [PMID: 30651602] (Bioassay, Mouse)	Bioassay	Mouse
Y Tang, S Xiong, P Yu, F Liu, L Cheng Direct Conversion of Mouse Fibroblasts into Neural Stem Cells by Chemical Cocktail Requires Stepwise Activation of Growth Factors and Nup210 Cell Rep, 2018-07-31;24(5):1355-1362.e3. 2018-07-31 [PMID: 30067988] (Bioassay, Mouse)	Bioassay	Mouse
YY Wang, S Ma, Q Chen, D Jiao, Y Yang In vivo selection with lentiviral expression of Bcl2T69A/S70A/S87Amutant in hematopoietic stem cell-transplanted mice Gene Ther., 2018-03-09;0(0):. 2018-03-09 [PMID: 29523881] (Bioassay, Mouse)	Bioassay	Mouse
J Du, X Wang, X Zhang, X Zhang, H Jiang DNER modulates the length, polarity and synaptogenesis of spiral ganglion neurons via the Notch signaling pathway Mol Med Rep, 2017-11-20;17(2):2357-2365. 2017-11-20 [PMID: 29207144] (Bioassay, Mouse)	Bioassay	Mouse
J Yang, C Wu, I Stefanescu, A Horowitz Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies J Vis Exp, 2017-04-22;0(122):. 2017-04-22 [PMID: 28518115] (Bioassay, Mouse)	Bioassay	Mouse
MM Mata-Miran, GJ Vazquez-Za, M Rojas-Lope, V Sanchez-Mo, DG Perez-Ishi, RJ Delgado-Ma Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells Biol. Res., 2017-04-04;50(1):14. 2017-04-04 [PMID: 28376862] (Bioassay, Mouse)	Bioassay	Mouse
G Valenti, HM Quinn, GJ Heynen, L Lan, JD Holland, R Vogel, A Wulf-Golde, W Birchmeier Cancer stem cells regulate cancer-associated fibroblasts via activation of Hedgehog signaling in mammary gland tumors Cancer Res, 2017-02-15;0(0):. 2017-02-15 [PMID: 28202523] (Bioassay, Mouse)	Bioassay	Mouse
Di Jiang miR-124 promotes the neuronal differentiation of mouse inner ear neural stem cells Int. J. Mol. Med, 2016-09-27;38(5):1367-1376. 2016-09-27 [PMID: 28025992] (Bioassay, Mouse)	Bioassay	Mouse
Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow Nat Cell Biol, 2016-09-19;18(10):1078-1089. 2016-09-19 [PMID: 27642788] (Bioassay, Mouse)	Bioassay	Mouse
Renalase expression by melanoma and tumor associated-macrophages promotes tumor growth through a STAT3-mediated mechanism Cancer Res, 2016-05-09;0(0):. 2016-05-09 [PMID: 27197188] (Bioassay, Mouse)	Bioassay	Mouse
Show All 13 Publications. Collapse Publications.

Array 8878-LF/CF

• LIF Antibodies
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• LIF Lysate
• LIF Proteins
• LIF ELISAs
• LIF Proteins and Enzymes