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Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF

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When Recombinant Mouse IL‑18 R alpha /IL-1 R5 Fc Chimera (Catalog # 9285‑BP) is coated at 2 μg/mL, 100 μL/well, Recombinant Mouse IL‑18/IL‑1F4 (Catalog # 9139‑IL) binds with a ED50 of 1.5-9 μg/mL.
When Recombinant Mouse IL‑18 R alpha /IL-1 R5 Fc Chimera (Catalog # 9285‑BP) is coated at 2 μg/mL, 100 μL/well, Recombinant Mouse IL‑18/IL‑1F4 (Catalog # 9139‑IL) binds with a ED50 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL‑18 R alpha /IL‑1 R5 Chimera is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Mouse IL-18 (Catalog # 9139-CL) that produces 50% of the optimal binding response is 1.5-9 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-18 R alpha/IL-1 R5 protein
Mouse IL-18 R alpha/IL-1 R5
(Lys20-Gly326)
Accession # Q61098		 IEGRDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Lys20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
90-106 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF

  • CD218a
  • CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1
  • IL-1 R5
  • IL-1 Rrp
  • IL-18 R alpha
  • IL-18 R1
  • IL18R alpha
  • IL18R1
  • IL-18Ra

Background

IL-18 Receptor alpha (IL-18 Ra), also known as IL-18 R1, IL-1 R5, and IL-1 Rrp, is a transmembrane component of the IL-18 Receptor (1). It associates with IL‑18 Rb/IL‑1 R7 to form the high affinity receptor complex for IL-18 and confer cellular responsiveness to IL-18 (2, 3). IL-18 is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. Mature mouse IL-18 Ra consists of a 307 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain (4). Within the ECD, mouse IL-18 Ra shares 56% and 82% aa sequence identity with human and rat IL-18 Ra, respectively. IL-18 Ra is widely expressed on epithelial cells as well as on B cells, NK cells, CD4+ and CD8+ T cells, and dendritic cells (5-8). Signaling through the IL-18 receptor promotes a Th1 biased immune response and NK cell cytolytic activity (6, 7, 9-11). It also contributes to cigarette smoke-induced airway inflammation and epithelial damage (12).
  1. Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
  2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  4. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
  5. Kunikata, T. et al. (1998) Cell Immunol. 189:135.
  6. Smeltz, R.B. et al. (2001) J. Exp. Med. 194:143.
  7. Chan, W.L. et al. (2001) J. Immunol. 167:1238.
  8. Kaser, A. et al. (2004) Blood 103:648.
  9. Tomura, M. et al. (1998) J. Immunol. 160:3759.
  10. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
  11. Hyodo, Y. et al. (1999) J. Immunol. 162:1662.
  12. Kang, M.J. et al. (2007) J. Immunol. 178:1948.

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Recombinant Mouse
IL-18 R alpha/IL-1 R5 ...
9285-BP
Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF
Species: Mu
Applications: Bioactivity

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