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Recombinant Human TIM-4 His-tag Protein, CF

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Recombinant Human TIM-4 (Catalog # 9407-TM)inhibits anti-CD3 antibody-induced proliferation of human T cells. The ED50 forthis effect is 0.06-0.36 μg/mL.
Recombinant Human TIM-4 (Catalog # 9407-TM)inhibits anti-CD3 antibody-induced proliferation of human T cells. The ED50 forthis effect is 0.06-0.36 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human TIM-4 His-tag Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.06-0.36 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human TIM-4 protein
Glu25-Gln314, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Glu25
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
77-90 kDa, reducing conditions
Publications
Read Publication using
9407-TM in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TIM-4 His-tag Protein, CF

  • FLJ27515
  • Smuckler
  • T-cell immunoglobulin and mucin domain containing 4
  • T-cell immunoglobulin and mucin domain-containing protein 4
  • T-cell membrane protein 4
  • TIM4
  • TIM-4
  • TIMD4
  • TIMD-4

Background

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1-3). The human TIM-4 cDNA encodes a 378 amino acid (aa) precursor that includes a 24 aa signal sequence, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1 or TIM-3. Within the ECD, human TIM-4 shares 35% and 23% aa sequence identity with TIM-1 and TIM-3, respectively. A TIM-2 ortholog has not been identified in human. The ECD of human TIM-4 shares 45% aa sequence identity with that of mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (5-7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (5).
  1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
  4. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494.
  5. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  6. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  7. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  8. Khademi, M. et al. (2004) J. Immunol. 172:7169.

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Recombinant Human
TIM-4 His-tag Protein ...
9407-TM
Recombinant Human TIM-4 His-tag Protein, CF
Species: Hu
Applications: Bioactivity

Publications for TIM-4 (9407-TM)(1)

We have publications tested in 1 confirmed species: N/A.

We have publications tested in 1 application: Bioassay.


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