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Recombinant Human TGF-beta 1 Protein

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Equivalent bioactivity of CHO-derived (Catalog # 240-B) and HEK293-derived (7754-BH) Recombinant Human TGF‑ beta 1 as measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cell. ...read more

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human TGF-beta 1 Protein Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.0400-0.200 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein
Ala279-Ser390
Accession #
N-terminal Sequence
Ala279
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TGFB1
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
24 kDa, non-reducing conditions
Publications
Read Publications using
240-B in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Purified recombinant human TGF-beta 1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, reconstitute 2 μg vials at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin. Reconstitute 10 μg or larger vials at 100 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin. 

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 1 Protein

  • CEDLAP
  • DPD1
  • latency-associated peptide
  • TGF beta
  • TGF beta1
  • TGFB
  • TGFB1
  • TGF-beta 1 protein
  • TGFbeta 1
  • TGF-beta 1
  • TGFbeta
  • TGF-beta-1
  • transforming growth factor beta-1
  • transforming growth factor, beta 1

Background

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non‑redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta  RIII) and endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).
  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

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Publications for TGF-beta 1 (240-B)(869)

We have publications tested in 14 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Equine, Feline, Fish, Mink, Porcine, Rabbit, Transgenic Mouse, Virus.

We have publications tested in 14 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), ELISA Capture, ELISA Developmet, ELISA Standard, Flow Cytometry, IHC-P, In Vivo, Stimulation, Surface Plasmon Resonance (SPR, Tissue Culture, Western Blot.


Filter By Application
Bioassay
(762)
Cell Culture
(53)
Differentiation
(5)
ELISA (Standard)
(7)
ELISA Capture
(1)
ELISA Developmet
(1)
ELISA Standard
(2)
Flow Cytometry
(1)
IHC-P
(1)
In Vivo
(17)
Stimulation
(6)
Surface Plasmon Resonance (SPR
(1)
Tissue Culture
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(572)
Mouse
(232)
Rat
(45)
Bovine
(1)
Canine
(7)
Chicken
(2)
Equine
(4)
Feline
(3)
Fish
(1)
Mink
(2)
Porcine
(4)
Rabbit
(4)
Transgenic Mouse
(5)
Virus
(1)
All Species
Showing Publications 1 - 10 of 869. Show All 869 Publications.
Publications using 240-B Applications Species
Koksalar Alkan, F;Caglayan, AB;Alkan, HK;Benson, E; Dual activity of Minnelide chemosensitize basal/triple negative breast cancer stem cells and reprograms immunosuppressive tumor microenvironment Scientific Reports 2024-01-01 [PMID: 39341857] (Bioassay, Human) Bioassay Human
Bette, M;Reinhardt, L;Gansukh, U;Xiang-Tischhauser, L;Meskeh, H;Di Fazio, P;Buchholz, M;Stuck, BA;Mandic, R; The Role of TGF-?1 and Mutant SMAD4 on Epithelial-Mesenchymal Transition Features in Head and Neck Squamous Cell Carcinoma Cell Lines Cancers 2024-09-16 [PMID: 39335144] (Bioassay, Human) Bioassay Human
Xuan, Z;Chen, C;Sun, H;Yang, K;Li, J;Fu, M;Bai, Y;Zheng, Z;Zhao, Y;Xu, C;Liu, B;Li, T;Shao, C; NDR1/FBXO11 promotes phosphorylation-mediated ubiquitination of ?-catenin to suppress metastasis in prostate cancer International journal of biological sciences 2024-09-16 [PMID: 39309441] (Bioassay, Human) Bioassay Human
Erler, P;Kurcon, T;Cho, H;Skinner, J;Dixon, C;Grudman, S;Rozlan, S;Dessez, E;Mumford, B;Jo, S;Boyne, A;Juillerat, A;Duchateau, P;Poirot, L;Aranda-Orgilles, B; Multi-armored allogeneic MUC1 CAR T cells enhance efficacy and safety in triple-negative breast cancer Science advances 2024-08-30 [PMID: 39213364] (Bioassay, Human) Bioassay Human
Jaroszewski, A;Geysels, RC;Volpini, X;Pellizas, CG;Motran, CC;Stempin, CC;Nicola, JP;Cheng, SY;Fozzatti, L; Anaplastic thyroid cancer cell-secreted TGF?1 plays a key role in inducing macrophage polarization of human monocytes American journal of cancer research 2024-07-15 [PMID: 39113863] (Bioassay, Human) Bioassay Human
Lim, YJ;Park, SA;Wang, D;Jin, W;Ku, WL;Zhang, D;Xu, J;Patiño, LC;Liu, N;Chen, W;Kazmi, R;Zhao, K;Zhang, YE;Sun, L;Chen, W; MicroRNA-19b exacerbates systemic sclerosis through promoting Th9 cells Cell reports 2024-07-30 [PMID: 39083380] (Bioassay, Human, Mouse) Bioassay Human, Mouse
Fesneau, O;Thevin, V;Pinet, V;Goldsmith, C;Vieille, B;M'Homa Soudja, S;Lattanzio, R;Hahne, M;Dardalhon, V;Hernandez-Vargas, H;Benech, N;Marie, JC; An intestinal TH17 cell-derived subset can initiate cancer Nature immunology 2024-07-26 [PMID: 39060651] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Weinberg, A;Johnson, M;Crotteau, M;Ghosh, D;Vu, T;Levin, MJ; Trained Immunity Generated by the Recombinant Zoster Vaccine Research square 2024-07-10 [PMID: 39041035] (Bioassay, Human) Bioassay Human
Patel, R;Cardona, CL;Angeles, E;Singh, G;Ashok, A;Teich, AF;Sproul, AA; Reduced SH3RF3 may protect against Alzheimer's disease by lowering microglial pro-inflammatory responses via modulation of JNK and NFkB signaling bioRxiv : the preprint server for biology 2024-06-28 [PMID: 38979369] (Bioassay, Human) Bioassay Human
Khanal, S;Liu, Y;Bamidele, AO;Wixom, AQ;Washington, AM;Jalan-Sakrikar, N;Cooper, SA;Vuckovic, I;Zhang, S;Zhong, J;Johnson, KL;Charlesworth, MC;Kim, I;Yeon, Y;Yoon, S;Noh, YK;Meroueh, C;Timbilla, AA;Yaqoob, U;Gao, J;Kim, Y;Lucien, F;Huebert, RC;Hay, N;Simons, M;Shah, VH;Kostallari, E; Glycolysis in hepatic stellate cells coordinates fibrogenic extracellular vesicle release spatially to amplify liver fibrosis Science advances 2024-06-28 [PMID: 38941469] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
Show All 869 Publications.

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Blogs on TGF-beta 1.

TGF-beta 1 - a versatile signaling molecule with roles in development and disease
The transforming growth factor-beta (TGF-beta) family consists of a wide variety of signaling proteins with roles in development. TGF-beta signaling controls growth, differentiation, and immune responses and is often misregulated in cancer. TGF-beta ...  Read full blog post.

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Bioinformatics

Gene Symbol TGFB1
Uniprot