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Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

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Recombinant human TGF-beta 1 (7754-BH) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL.
Recombinant human TGF-beta 1 (7754-BH) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
7754-BH-005/CF
Carrier Free
7754-BH-025/CF
Carrier Free
7754-BH-100/CF
Carrier Free
7754-BH-500/CF
Carrier Free
7754-BH-01M/CF
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Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human TGF-beta 1 protein
Ala279-Ser390
Accession #
N-terminal Sequence
Ala279
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TGFB1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
11 kDa, reducing conditions
Publications
Read Publications using
7754-BH in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

  • CEDLAP
  • DPD1
  • latency-associated peptide
  • TGF beta
  • TGF beta1
  • TGFB
  • TGFB1
  • TGF-beta 1 protein
  • TGFbeta 1
  • TGF-beta 1
  • TGFbeta
  • TGF-beta-1
  • transforming growth factor beta-1
  • transforming growth factor, beta 1

Background

TGF‑ beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF‑ beta superfamily that share a characteristic cystine knot structure (1‑7). TGF‑ beta 1, ‑2 and ‑3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non‑redundant functions; for TGF‑ beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide-linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta  RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta  RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).
  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press p. 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

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Recombinant Human
TGF-beta 1 (Human Cel ...
7754-BH
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
Species: Hu
Applications: Bioactivity

Publications for TGF-beta 1 (7754-BH)(69)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Complex Species Category, Transgenic Mouse.

We have publications tested in 2 applications: Bioassay, Cell Culture.


Filter By Application
Bioassay
(65)
Cell Culture
(4)
All Applications
Filter By Species
Human
(57)
Mouse
(9)
Rat
(1)
Complex Species Category
(1)
Transgenic Mouse
(2)
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Showing Publications 1 - 10 of 69. Show All 69 Publications.
Publications using 7754-BH Applications Species
Marimoutou, M;Patel, V;Kim, JH;Schaible, N;Alvarez, J;Hughes, J;Obermok, M;Rodríguez, CI;Kallarakal, T;Suki, B;Amin, K;Krishnan, R;Behrsing, HP; The Fibrotic Phenotype of Human Precision-Cut Lung Slices Is Maintained after Cryopreservation Toxics 2024-08-30 [PMID: 39330565] (Bioassay, Human) Bioassay Human
Kurosawa, T;Ikemoto-Uezumi, M;Yoshimoto, Y;Minato, K;Kaji, N;Chaen, T;Hase, E;Minamikawa, T;Yasui, T;Horiguchi, K;Iino, S;Hori, M;Uezumi, A; Tissue-specific functions of MSCs are linked to homeostatic muscle maintenance and alter with aging Aging cell 2024-09-25 [PMID: 39323233] (Bioassay, Mouse) Bioassay Mouse
Hapeman, JD;Galwa, R;Carneiro, CS;Nedelcu, AM; In vitro evidence for the potential of EGFR inhibitors to decrease the TGF-?1-induced dispersal of circulating tumour cell clusters mediated by EGFR overexpression Scientific reports 2024-08-28 [PMID: 39198539] (Bioassay, Human) Bioassay Human
Li, H;Wang, G;Zhao, G;Liu, H;Liu, L;Cao, Y;Li, C; TGF-?1 maintains the developmental potential of embryonic submandibular gland epithelia separated with mesenchyme Heliyon 2024-07-15 [PMID: 39040362] (Bioassay, Mouse) Bioassay Mouse
Law, C;Wacleche, VS;Cao, Y;Pillai, A;Sowerby, J;Hancock, B;Horisberger, A;Bracero, S;Skidanova, V;Li, Z;Adejoorin, I;Dillon, E;Benque, IJ;Nunez, DP;Simmons, DP;Keegan, J;Chen, L;Baker, T;Brohawn, PZ;Al-Mossawi, H;Hao, LY;Jones, B;Rao, N;Qu, Y;Alves, SE;Accelerating Medicines Partnership: RA/SLE Network, ;Jonsson, AH;Shaw, KS;Vleugels, RA;Massarotti, E;Costenbader, KH;Brenner, MB;Lederer, JA;Hultquist, JF;Choi, J;Rao, DA; Interferon subverts an AHR-JUN axis to promote CXCL13+ T cells in lupus Nature 2024-07-10 [PMID: 38987586] (Bioassay, Human) Bioassay Human
Guo, F;Zhang, L;Yu, Y;Gong, L;Tao, S;Werder, RB;Mishra, S;Zhou, Y;Anamika, WJ;Lao, T;Inuzuka, H;Zhang, Y;Pham, B;Liu, T;Tufenkjian, TS;Richmond, BW;Wei, W;Mou, H;Wilson, AA;Hu, M;Chen, W;Zhou, X; Identification of a distal enhancer regulating hedgehog interacting protein gene in human lung epithelial cells EBioMedicine 2024-02-27 [PMID: 38417378] (Bioassay, Human) Bioassay Human
Ranjbarvaziri, S;Zeng, A;Wu, I;Greer-Short, A;Farshidfar, F;Budan, A;Xu, E;Shenwai, R;Kozubov, M;Li, C;Van Pell, M;Grafton, F;MacKay, CE;Song, X;Priest, JR;Argast, G;Mandegar, MA;Hoey, T;Yang, J; Targeting HDAC6 to treat heart failure with preserved ejection fraction in mice Nature communications 2024-02-26 [PMID: 38409164] (Bioassay, Human) Bioassay Human
Cao, S;Pan, Y;Terker, AS;Arroyo Ornelas, JP;Wang, Y;Tang, J;Niu, A;Kar, SA;Jiang, M;Luo, W;Dong, X;Fan, X;Wang, S;Wilson, MH;Fogo, A;Zhang, MZ;Harris, RC; Epidermal growth factor receptor activation is essential for kidney fibrosis development Nature communications 2023-11-14 [PMID: 37963889] (Bioassay, Mouse) Bioassay Mouse
Katagata, M;Okayama, H;Nakajima, S;Saito, K;Sato, T;Sakuma, M;Fukai, S;Endo, E;Sakamoto, W;Saito, M;Saze, Z;Momma, T;Mimura, K;Kono, K; TIM-3 Expression and M2 Polarization of Macrophages in the TGF?-Activated Tumor Microenvironment in Colorectal Cancer Cancers 2023-10-11 [PMID: 37894310] (Cell Culture) Cell Culture
Hapeman, JD;Carneiro, CS;Nedelcu, AM; A model for the dissemination of circulating tumour cell clusters involving platelet recruitment and a plastic switch between cooperative and individual behaviours BMC ecology and evolution 2023-08-21 [PMID: 37605189] (Bioassay, Human) Bioassay Human
Show All 69 Publications.

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Bioinformatics

Gene Symbol TGFB1
Uniprot