Home » OLFM4 » OLFM4 Proteins » Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF

 Submit a Review

Images

 
(
Enlarge)
2 μg/lane of Recombinant Human Olfactomedin-4/OLFM4 His-tag (Catalog # 10261-OL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 66-75 kDa on reducing gel.
2 μg/lane of Recombinant Human Olfactomedin-4/OLFM4 His-tag (Catalog # 10261-OL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the spreading of NIH3T3 mouse embryonic fibroblast cells Recombinant Human Olfactomedin-4/OLFM4 His-tag (Catalog # 10261-OL) immobilized at 0.5-1 μg/mL will significantly support cell spreading.
Source
Chinese Hamster Ovary cell line, CHO-derived human Olfactomedin-4/OLFM4 protein
Asp21-Gln510, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Asp21
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW

56 kDa

.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
66-75 kDa, under reducting conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF

  • Antiapoptotic protein GW112
  • bA209J19.1
  • GC1
  • G-CSF-stimulated clone 1 protein
  • GW112
  • GW112hOLfD
  • hGC-1
  • KIAA4294
  • olfactomedin 4
  • Olfactomedin-4
  • OlfD
  • OLFM4
  • OLM4
  • UNQ362

Background

Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF stimulated clone-1) is an approximately 64-72 kDa secreted glycoprotein that is a member of the Olfactomedin/Noelin family of protein (1-3). It is expressed in epithelial cells of the prostate, small intestine, colon, bone marrow, and in several cancers (4-7), and is up-regulated in epithelial cells during inflammation (8). Mature OLFM4 is a 490 amino acid (aa) protein that is secreted mainly in a polymeric form which is held together by disulfide bonds and carbohydrate interactions (2). Mature human OLFM4 shares 71% aa sequence identity with mature mouse and rat OLFM4. Olfactomedin-4 (OLFM4) interacts with cell surface lectins and cadherins, which helps cell adhesion and cell spreading of NIH3T3 and 293T/17 cells (9). OLFM4 plays a critical role in regulating progression of prostate cancer and is also novel biomarker for triple-negative breast cancer (10-11).
  1. Zhang, J. et al. (2002) Gene 283:83.
  2. Grover, P.K. et al. (2010) Cancer Metastasis Rev. 29:761.
  3. Guette, C. et al. (2015) Proteomics Clin. Appl. 9:58.
  4. Zhang, X. et al. (2004) Cancer Res. 64:2474.
  5. Kobayashi, D. et al. (2007) Cancer Sci. 98:334.
  6. Koshida, S. et al. (2007) Cancer Sci. 98:315.
  7. Yu, L. et al. (2011) Int. J. Gynecol. Cancer 21:367.
  8. Shinozaki, S. et al. (2001) Gut 48:623.
  9. Liu, W. et al. (2006) Exp. Cell Res. 312:1785.
  10. Xiong, B. et al. (2017) Biomed. Pharmacother. 86:67.
  11. Li, H. et al. (2015) Sci. Rep. 5:16974.

Customers Who Viewed This Item Also Viewed...

G-CSF Antibody
(67604) [Unconjugated
MAB414
Neutralization G-CSF Antibody (67604) [Unconjugated]
(60 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Neut, WB
SLC25A18 Antibody
NBP3-17540
Western Blot SLC25A18 Antibody
Immunocytochemistry/ Immunofluorescence SLC25A18 Antibody
Species: Hu
Applications: ICC/IF, WB
GCAP1 Antibody
NBP2-55158
Immunohistochemistry-Paraffin GCAP1 Antibody
Immunohistochemistry-Paraffin GCAP1 Antibody
Immunohistochemistry-Paraffin GCAP1 Antibody
(1 Publication)
Species: Hu, Pm
Applications: IHC, IHC-Fr, IHC-P
GUCY2D Antibody
NBP3-12211
Immunohistochemistry-Paraffin GUCY2D Antibody
Unknown GUCY2D Antibody
Western Blot GUCY2D Antibody
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Reg4 Antibody
[Unconjugated]
AF1379
Immunohistochemistry Reg4 Antibody [Unconjugated]
(1 Review)
(9 Publications)
Species: Hu
Applications: IHC, WB
GRIM19 Antibody
NBP1-90050
Immunohistochemistry-Paraffin GRIM19 Antibody
Immunohistochemistry-Paraffin GRIM19 Antibody
Immunohistochemistry-Paraffin GRIM19 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
GCAP2 Antibody
NBP2-68721
Western Blot GCAP2 Antibody
Immunohistochemistry-Paraffin GCAP2 Antibody
(1 Publication)
Species: Hu, Pm
Applications: IHC, IHC-Fr, IHC-P, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
G-CSF Antibody
(67604) [Unconjugated
MAB414
Neutralization G-CSF Antibody (67604) [Unconjugated]
(60 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Neut, WB
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Rhodopsin Antibody
(4D2)
NBP2-59690
Immunohistochemistry-Frozen Rhodopsin Antibody (4D2)
Western Blot Rhodopsin Antibody (4D2)
Immunohistochemistry Rhodopsin Antibody (4D2)
(1 Review)
(6 Publications)
Species: Am, Av, Fi, Ma, Ze
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IP, WB
NFkB p105/p50
Antibody (2J10D7) - B ...
NB100-56583
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
(1 Review)
(5 Publications)
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
EGF [Unconjugated]
236-EG
SDS-PAGE EGF [Unconjugated]
Bioactivity EGF [Unconjugated]
Cell Culture EGF [Unconjugated]
(834 Publications)
Species: Hu
Applications: BA
LIGHT/TNFSF14
[Unconjugated]
664-LI
SDS-PAGE LIGHT/TNFSF14 [Unconjugated]
Bioactivity LIGHT/TNFSF14 [Unconjugated]
(21 Publications)
Species: Hu
Applications: BA
CEACAM5/CD66e
Antibody (CEA31) - Az ...
NBP2-34594
Immunohistochemistry-Paraffin CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free
Immunohistochemistry-Paraffin CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free
Immunohistochemistry-Paraffin CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free
(2 Reviews)
Species: Hu, Pm
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P
CEACAM7 Antibody
[Unconjugated]
AF4478
Immunohistochemistry CEACAM7 Antibody [Unconjugated]
Western Blot CEACAM7 Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: IHC, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(811 Publications)
Species: Hu
Applications: BA
Neurofascin
Antibody
AF3235
Western Blot Neurofascin Antibody
Immunohistochemistry Neurofascin Antibody
(1 Review)
(32 Publications)
Species: Hu, Mu, Rt
Applications: IHC, WB

Publications for OLFM4 (10261-OL) (0)

There are no publications for OLFM4 (10261-OL).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for OLFM4 (10261-OL) (0)

There are no reviews for OLFM4 (10261-OL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for OLFM4 (10261-OL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional OLFM4 Products

Blogs on OLFM4

There are no specific blogs for OLFM4, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF and receive a gift card or discount.


Bioinformatics

Uniprot