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GCAP2 Antibody

Images

 
Western Blot: GCAP2 Antibody [NBP2-68721] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunohistochemistry-Paraffin: GCAP2 Antibody [NBP2-68721] - Staining of human retina shows strong cytoplasmic positivity in cones and outer plexiform layer.

Product Details

Summary
Reactivity Hu, Pm, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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GCAP2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GQEFSWEEAEAAGEIDVAELQEWYKKFVMECPSGTLFMHEFKRFFKVTDDEEASQYVEGMFRAFDKNG
Predicted Species
Mouse (90%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GUCA1B
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Frozen Reported in scientific literature (PMID:35024589)
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
Recommended conditions for IHC,Retrieval method: HIER pH6.
Control Peptide
GCAP2 Recombinant Protein Antigen (NBP2-68721PEP)
Publications
Read Publication using
NBP2-68721 in the following applications:

Reactivity Notes

Use in Monkey reported in scientific literature (PMID:35024589) .

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for GCAP2 Antibody

  • DKFZp686E1183
  • GCAP 2
  • GCAP2guanylate cyclase-activating protein, photoreceptor 2
  • guanylate cyclase activator 1B (retina)
  • Guanylate cyclase activator 1B
  • guanylyl cyclase-activating protein 2
  • GUCA2
  • RP48

Background

Guanylate cyclase-activating proteins (GCAPs) are calcium binding proteins that belong to the calmodulin superfamily. GCAPs without calcium are responsible for activation of photoreceptor guanylate cyclase during light adaptation. Studies have shown that the addition or subtraction of Ca2+ results in major conformational changes and the activation/deactivation of GCAP 1 and GCAP 2. It has been demonstrated that both GCAP 1 and 2 act on guanylate cyclase similarly and have approximately 50% homology between the two forms. GCAP-1 is predominantly localized in the photoreceptor outer segments, while GCAP 2 is found in retina samples.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GCAP2 Antibody (NBP2-68721)(1)

We have publications tested in 1 confirmed species: Monkey.

We have publications tested in 1 application: IHC-Fr.


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IHC-Fr
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GUCA1B