Reactivity | HuSpecies Glossary |
Applications | Enzyme Activity |
Format | Carrier-Free |
Additional Information | FUT3 has been formulated so that it can be used in the cell surface glycoengineering of living cells, and does not affect cell viability or native phenotype apart from the intended impact on cell glycobiology. |
Details of Functionality | Measured by its ability to transfer fucose from GDP-fucose to N-Acetyllactosamine The specific activity is >25 pmol/min/µg, as measured under the described conditions. |
Source | Mouse myeloma cell line, NS0-derived human Fucosyltransferase 3/FUT3 protein Arg35-Thr361, with an N-terminal 6-His tag |
Accession # | |
N-terminal Sequence | His |
Protein/Peptide Type | Recombinant Enzymes |
Gene | FUT3 |
Purity | >90%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Endotoxin Note | <1.0 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 39 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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SDS-PAGE | 41 kDa, reducing conditions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
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Purity | >90%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
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Assay Procedure |
*Derived from the phosphate standard curve using linear or 4-parameter fitting and adjusted for Control. Per Reaction:
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Because N-, O-glycans and glycolipids are frequently fucosylated at terminal sites, fucose is often found to be essential for sugar epitope and lectin ligand generation. Well-known fucose containing structures include Lewis structures and ABO blood group antigens. Lewis structures are key elements involved in leukocyte homing and extravasation process and thus are essential for lymphocyte maturation and natural defense functions. Fucose containing glycans also play essential roles in cell signaling and development. So far, more than 10 fucosyltransferases have been cloned from the human genome (1). FUT1 and FUT2 are
alpha 1-2 fucosyltransferases and are responsible for ABO blood group antigen synthesis. FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are responsible for Lewis structure generation through their alpha 1-3 or alpha 1-4 fucosyltransferases activities. FUT3, also known as Lewis blood group fucosyltransferase, is unique by having both strong
alpha 1‑3 and alpha 1‑4 fucosyltransferase activities (2). FUT3 has high homology with FUT5 and FUT6 due to gene duplication. FUT7 is exclusively responsible for biosynthesis of sialyl Lewis X epitope in leukocytes and high endothelial venule cells (3). FUT8 is an alpha 1-6 fucosyltransferase that adds a fucose to the chitobiose core of N‑glycans (4). Predicted as type II transmembrane proteins and Golgi enzymes, some of the fucosyltransferases can also be found in plasma. R&D Systems recombinant human FUTs correspond to the luminal domains. The enzymatic activity of recombinant human FUT3 was determined using a phosphatase‑coupled glycosyltransferase assay (5).
Publication using 4950-GT | Applications | Species |
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Su, T;Chua, WZ;Liu, Y;Fan, J;Tan, SY;Yang, DW;Sham, LT; Rewiring the pneumococcal capsule pathway for investigating glycosyltransferase specificity and genetic glycoengineering Science advances 2023-09-08 [PMID: 37672581] (Enzyme Activity, Bacteria) | Enzyme Activity | Bacteria |
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