I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?

Name: Recombinant Human FGF basic/FGF2/bFGF (157 aa) Protein
Brand: Novus Biologicals
SKU: 234-FSE
Rating: 5 (1 reviews)

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Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our  NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	Bioactivity

Catalog# & Formulation	Size	Price
Carrier Free 234-FSE-025/CF

Details of Functionality	Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 0.5‑2.5 ng/mL.
Source	E. coli-derived human FGF basic/FGF2/bFGF protein Gly132-Ser288
Accession #	NP_001997
N-terminal Sequence	Gly132
Protein/Peptide Type	Recombinant Proteins
Gene	FGF2
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note	<0.10 EU per 1 μg of the protein by the LAL method.

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Recombinant Human FGF basic/FGF2/bFGF (157 aa) Protein

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Publications using 234-FSE	Applications	Species
Cervantes-Villagrana, RD;Mendoza, V;Hinck, CS;de la Fuente-León, RL;Hinck, AP;Reyes-Cruz, G;Vázquez-Prado, J;López-Casillas, F; Betaglycan sustains HGF/Met signaling in lung cancer and endothelial cells promoting cell migration and tumor growth Heliyon 2024-05-15 [PMID: 38756586] (Bioassay, Mouse, Porcine)	Bioassay	Mouse, Porcine
Shabani, K;Pigeon, J;Benaissa Touil Zariouh, M;Liu, T;Saffarian, A;Komatsu, J;Liu, E;Danda, N;Becmeur-Lefebvre, M;Limame, R;Bohl, D;Parras, C;Hassan, BA; The temporal balance between self-renewal and differentiation of human neural stem cells requires the amyloid precursor protein Science advances 2023-06-16 [PMID: 37327344] (Bioassay, Human)	Bioassay	Human
F Hermans, L Hemeryck, C Bueds, M Torres Per, S Hasevoets, H Kobayashi, D Lambrechts, I Lambrichts, A Bronckaers, H Vankelecom Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development Stem Cell Reports, 2023-04-20;18(5):1166-1181. 2023-04-20 [PMID: 37084723] (Bioassay, Mouse)	Bioassay	Mouse
JH Kim, IR Lim, CY Park, HJ Joo, JM Noh, SC Choi, SJ Hong, DS Lim Thymosin &beta4-Enhancing Therapeutic Efficacy of Human Adipose-Derived Stem Cells in Mouse Ischemic Hindlimb Model Int J Mol Sci, 2020-03-21;21(6):. 2020-03-21 [PMID: 32245208] (Bioassay, Human)	Bioassay	Human
JH Qi, B Bell, R Singh, J Batoki, A Wolk, A Cutler, N Prayson, M Ali, H Stoehr, B Anand-Apte Sorsby Fundus Dystrophy Mutation in Tissue Inhibitor of Metalloproteinase 3 (TIMP3) promotes Choroidal Neovascularization via a Fibroblast Growth Factor-dependent Mechanism Sci Rep, 2019-11-22;9(1):17429. 2019-11-22 [PMID: 31757977] (Bioassay, Human)	Bioassay	Human
T Menguy, A Briaux, E Jeunesse, J Giustinian, A Calcei, T Guyon, J Mizrahi, H Haegel, V Duong, V Soler, P Brousset, A Bensussan, I Raymond Le, P Le Bouteil Anti-CD160, Alone or in Combination With Bevacizumab, Is a Potent Inhibitor of Ocular Neovascularization in Rabbit and Monkey Models Invest. Ophthalmol. Vis. Sci., 2018-06-01;59(7):2687-2698. 2018-06-01 [PMID: 29860455] (In Vivo, Human)	In Vivo	Human
M Li, X Zhao, W Wang, H Shi, Q Pan, Z Lu, SP Perez, R Suganthan, C He, M Bjørås, A Klungland Ythdf2-mediated m6A mRNA clearance modulates neural development in mice Genome Biol., 2018-05-31;19(1):69. 2018-05-31 [PMID: 29855337] (Bioassay, Mouse)	Bioassay	Mouse
Kjartansdottir K, Reda A, Panula S, Day K, Hultenby K, Soder O, Hovatta O, Stukenborg J A Combination of Culture Conditions and Gene Expression Analysis Can Be Used to Investigate and Predict hES Cell Differentiation Potential towards Male Gonadal Cells. PLoS ONE, 2015-12-02;10(12):e0144029. 2015-12-02 [PMID: 26630562] (Bioassay, Human)	Bioassay	Human
Wollin L, Maillet I, Quesniaux V, Holweg A, Ryffel B Antifibrotic and anti-inflammatory activity of the tyrosine kinase inhibitor nintedanib in experimental models of lung fibrosis. J Pharmacol Exp Ther, 2014-02-20;349(2):209-20. 2014-02-20 [PMID: 24556663] (Bioassay, Human)	Bioassay	Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)	Bioassay	Human
Ghosh JG, Shenoy AK, Clark JI Interactions between important regulatory proteins and human alphaB crystallin. Biochemistry, 2007-05-08;46(21):6308-17. 2007-05-08 [PMID: 17487982] (ELISA-Based Protein Pin Array, Human)	ELISA-Based Protein Pin Array	Human
Li F, Shetty AK, Sugahara K Neuritogenic activity of chondroitin/dermatan sulfate hybrid chains of embryonic pig brain and their mimicry from shark liver. Involvement of the pleiotrophin and hepatocyte growth factor signaling pathways. J. Biol. Chem., 2006-12-04;282(5):2956-66. 2006-12-04 [PMID: 17145750] (Bioassay, Fish - Prionace glauca (Blue Shark))	Bioassay	Fish - Prionace glauca (Blue Shark)
Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential. Am. J. Pathol., 2006-10-01;169(4):1167-82. 2006-10-01 [PMID: 17003476] (Bioassay, Human)	Bioassay	Human
Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes. Blood, 2006-08-01;108(3):928-35. 2006-08-01 [PMID: 16861346] (Bioassay, Human)	Bioassay	Human
Show All 14 Publications. Collapse Publications.

	Verified Customer 09/27/2023
Application:	Stem/Immune cell maintenance or differentiation
Species:

Gene Symbol	FGF2
Uniprot	NP_001997()