Recombinant Human CD79A Protein, CF Summary
Details of Functionality |
Bioassay data are not available. |
Source |
Mouse myeloma cell line, NS0-derived human CD79A protein Leu33-Arg143, with a C-terminal 6-His tag |
Accession # |
|
N-terminal Sequence |
Leu33 |
Protein/Peptide Type |
Innovator Recombinant Proteins |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
13 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
38-43 kDa, reducing conditions |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS and EDTA. |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions |
Reconstitute at 250 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human CD79A Protein, CF
Background
CD79A (also known as Mb-1, Ig alpha and B cell
antigen receptor complex-associated protein alpha-chain) is a 30-40 kDa member
of the Ig-Superfamily. It is expressed on B cells, and forms a covalent
heterodimer with CD79B. Heterodimers of CD79A and CD79B interacts noncovalently
with membrane Ig, forming the B cell antigen receptor (BCR) (1). Within this
complex, membrane Ig detects antigen while CD79AB initiates signaling (1).
CD79A is essential for the differentiation of pre-B cells, and the pre-BCR
regulates the surface expression of IL-7R (2). Mature human CD79A is a type I
transmembrane glycoprotein. It contains an extracellular region (aa 33-143)
with one C2-type Ig-like domain (aa 33-116), and an ITAM-containing cytoplasmic
domain (aa 177-205) (3). Within the ECD, human CD79A shares 57% amino acid sequence
identity with mouse and rat CD79A.
-
Tseng, J. et al. (1997) Blood, 89:1513.
- Kurosaki, T. (2000) Current opinion in immunology, 12:276.
- Radaev, Sergei et al. (2010) Structure 18:934.
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