Recombinant Human ADAM22 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

A disintegrin and metalloproteinase 22 (ADAM22), also known as MDC2, is an 85-90 kDa transmembrane member of the M12B family of peptidases (1). Human ADAM22 contains an N-terminal 197 amino acid (aa) cleavable proregion and a 514 aa extracellular domain (ECD). The ECD contains a nonfunctional metalloprotease domain, an integrin‑binding disintegrin domain, a cysteine‑rich region, and one EGF-like domain (2). The cytoplasmic portion contains two phosphorylation motifs which bind to 14-3-3 proteins to mediate cell adhesion (3, 4). Alternative splicing generates multiple isoforms with a variety of insertions, deletions, and substitutions in the cytoplasmic region (2, 5, 6). Within the peptidase-like and disintegrin domains, human ADAM22 shares 96% sequence identity with mouse and rat ADAM22. ADAM22 is expressed on neurons, astrocytes, and Schwann cells in the cerebral cortex, hippocampus, and cerebellum and also on some low-grade gliomas (5, 7-9). It can be up-regulated in endocrine therapy-resistant breast cancer, and it contributes to tumor cell migration (10). It mediates cell adhesion through binding to Integrins alpha 6, alpha 9, and beta 3 (7). ADAM22 is localized to post-synaptic densities (11). It binds the secreted protein LGI1 which functions as a bridge to link ADAM22 with pre-synaptically expressed ADAM23 (9). The interaction of LGI1 with ADAM22 promotes AMPA receptor expression in hippocampal neurons and strengthens excitatory synapses (9). In axonal juxtaparanodes, ADAM22 is a component of the voltage-gated Kv1 potassium channel that responds to LGI1 (12). It also colocalizes with Kv1 channels in basket cell interneuron terminals of the cerebellum (12). ADAM22 additionally binds to Schwann cell derived LGI4 and is required for LGI4 to promote axon myelination (8). Mice lacking ADAM22 expression exhibit hypomyelination and profound ataxia (13).

Array 4908-AD

• ADAM22 Antibodies
• ADAM22 ELISA Kit
• ADAM22 Protein
• ADAM22 Proteins and Enzyme