DLEC/CLEC4C/BDCA-2 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Dendritic cell lectin (DLEC), also known as BDCA-2, CD303, HECL, and CLEC4C/CLECSF11/CLECSF7, is a 38 kDa type II transmembrane protein in the C-type lectin family (1). Mature human DLEC consists of a 21 amino acid (aa) cytoplasmic domain, a 23 aa transmembrane segment, and a 169 aa extracellular domain (ECD) that contains a juxtamembrane neck region and one carbohydrate recognition domain (CRD) (2, 3). Alternate splicing may generate multiple isoforms that lack the transmembrane segment and/or portions of the cytoplasmic, neck, and CRD regions (2-4). An ortholog of human DLEC has not been described in mouse or rat. DLEC expression is restricted to plasmacytoid dendritic cells (pDC) and is downregulated during their maturation (2, 3, 5). pDC play a role in the innate immune response by producing IFN-alpha / beta following exposure to TLR7 and TLR9 agonists such as microbial CpG DNA (3, 5-8). Antibody ligation of DLEC on pDC attenuates the CpG-stimulated production of interferons as well as a Th1 biased response (3, 5-9). DLEC interactions with HIV-1 gp120 and hepatitis B virus soluble antigen may therefore limit the pDC antiviral response (10, 11). Similar to other C-type lectins, DLEC can mediate antigen uptake for MHC loading and presentation to T cells (3, 12). Crosslinking of DLEC on CpG-stimulated pDC inhibits pDC maturation and induces tyrosine phosphorylation on multiple proteins involved in B cell receptor signaling and endocytosis (5, 7, 8). These functions require the association of DLEC with the ITAM-containing Fc epsilon RI gamma chain (7, 8).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Macaca mulatta (Rhesus Macaque).

We have publications tested in 3 applications: IHC, IHC-Fr, Immunohistochemistry.

Showing Publications 1 - 7 of 7.

Publications using AF1376	Applications	Species
Warner van Dijk, FA;Tong, O;O'Neil, TR;Bertram, KM;Hu, K;Baharlou, H;Vine, EE;Jenns, K;Gosselink, MP;Toh, JW;Papadopoulos, T;Barnouti, L;Jenkins, GJ;Sandercoe, G;Haniffa, M;Sandgren, KJ;Harman, AN;Cunningham, AL;Nasr, N; Characterising plasmacytoid and myeloid AXL+ SIGLEC-6+ dendritic cell functions and their interactions with HIV PLoS pathogens 2024-06-26 [PMID: 38924030] (Immunohistochemistry, Human)	Immunohistochemistry	Human
F Bayerl, DA Bejarano, G Bertacchi, AC Doffin, E Gobbini, M Hubert, L Li, P Meiser, AM Pedde, W Posch, L Rupp, A Schlitzer, M Schmitz, BU Schraml, S Uderhardt, J Valladeau-, D Wilflingse, V Zaderer, JP Böttcher Guidelines for visualization and analysis of DC in tissues using multiparameter fluorescence microscopy imaging methods Oncogene, 2023-01-09;0(0):e2249923. 2023-01-09 [PMID: 36623939] (IHC, Human)	IHC	Human
I Plesca, I Benešová, C Beer, U Sommer, L Müller, R Wehner, M Heiduk, D Aust, G Baretton, MP Bachmann, A Feldmann, J Weitz, L Seifert, AM Seifert, M Schmitz Clinical Significance of Tumor-Infiltrating Conventional and Plasmacytoid Dendritic Cells in Pancreatic Ductal Adenocarcinoma Cancers, 2022-02-26;14(5):. 2022-02-26 [PMID: 35267524] (IHC, Human)	IHC	Human
MY Lee, AA Upadhyay, H Walum, CN Chan, RA Dawoud, C Grech, JL Harper, KA Karunakara, SA Nelson, EA Mahar, KL Goss, DG Carnathan, B Cervasi, K Gill, GK Tharp, ER Wonderlich, V Velu, SM Barratt-Bo, M Paiardini, G Silvestri, JD Estes, SE Bosinger Tissue-specific transcriptional profiling of plasmacytoid dendritic cells reveals a hyperactivated state in chronic SIV infection PloS Pathogens, 2021-06-28;17(6):e1009674. 2021-06-28 [PMID: 34181694] (IHC, Macaca mulatta (Rhesus Macaque))	IHC	Macaca mulatta (Rhesus Macaque)
Zehua Ma, Xiankui Cheng, Ting Yue, Xun Shangguan, Zhixiang Xin, Weiwei Zhang et al. Immune infiltration phenotypes of prostate adenocarcinoma and their clinical implications Cancer Medicine 2021-08-01 [PMID: 34128342]
Hirbod T, Kaldensjo T, Broliden K In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa. PLoS ONE, 2011-09-30;6(9):e25551. 2011-09-30 [PMID: 21984929] (IHC-Fr, Human)	IHC-Fr	Human
Tove Kaldensjö, Pernilla Petersson, Anna Tolf, Gareth Morgan, Kristina Broliden, Taha Hirbod Detection of Intraepithelial and Stromal Langerin and CCR5 Positive Cells in the Human Endometrium: Potential Targets for HIV Infection PLoS ONE 2011-06-29 [PMID: 21738639]

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Santhosh Sivajothi	IHC-P	Human	05/17/2018	View Summary Application Immunohistochemistry-Paraffin Sample Tested Human lymph node Species Human Lot IDL0217051 Comments Comments Heat mediated antigen retrieval performed in citrate buffer for 20 minutes (pH 6.0)

Array AF1376

• DLEC/CLEC4C/BDCA-2 Antibodies
• DLEC/CLEC4C/BDCA-2 Proteins and Enzyme