| Reactivity | FeSpecies Glossary |
| Applications | Bioactivity |
| Format | Carrier-Free |
| Details of Functionality | Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 1-4 µg/mL in the presence of 20 ng/mL of rhFas Ligand. |
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| Source | Mouse myeloma cell line, NS0-derived feline Fas/TNFRSF6/CD95 protein
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| Accession # | |||||||
| N-terminal Sequence | Ala25 |
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| Structure / Form | Disulfide-linked homodimer |
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| Protein/Peptide Type | Recombinant Proteins |
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| Gene | FAS |
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| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
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| Endotoxin Note | <0.01 EU per 1 μg of the protein by the LAL method. |
| Dilutions |
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| Theoretical MW | 43 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE | 59-61 kDa, reducing conditions |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Reconstitution Instructions | Reconstitute at 100 μg/mL in sterile PBS. |
Feline Fas (fibroblast associated; also named CD95 and APO-1) is a 45 kDa type I transmembrane (TM) glycoprotein that is a member of the TNF receptor superfamily (1 - 3). The family contains about 30 members, and is characterized by the presence of at least one cysteine-rich domain that contains multiple intrachain disulfide bonds. In general, the superfamily is divided into cytoplasmic death domain (DD) containing, and non-DD containing receptors (3). Feline Fas is synthesized as a 314 amino acid (aa) precursor that contains a 24 aa signal sequence, a 148 aa extracellular region, a 16 aa TM segment and a 126 aa cytoplasmic tail (4). The extracellular region contains four potential N-linked glycosylation sites plus two distinct cysteine-rich domains of approximately 40 aa each; the cytoplasmic tail shows a 45 aa DD. The extracellular region of feline Fas shares 68%, 65%, 53%, and 58% aa sequence identity to porcine, human, mouse and rat Fas, respectively. There are five alternate splice forms of feline Fas, which vary from 132 aa to 209 aa in length. All utilize exons 1 - 3 (aa 1 - 111) and all are missing the transmembrane segment of the full length form (5). Circulating Fas is reported to be both a dimer and trimer at low ng/mL concentrations. The ligand for Fas is FasL, and Fas ligation activates both the MEK cascade and FADD/caspase-8 pathway (7).
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| Gene Symbol | FAS |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Representative IF of cerebellar sections at P10 using an anti-CD95/Fas antibody (green), an anti-calbindin antibody (red) to highlight Purkinje cells. For IF, Hoechst dye (blue) was used to mark nuclei. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-017-0838-1), licensed under a CC-BY license](https://images.novusbio.com/images/Fas-Receptor-TNFRSF6-CD95-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13550-img0008.jpg "Immunocytochemistry/Immunofluorescence: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Representative IF of cerebellar sections at P10 using an anti-CD95/Fas antibody (green), an anti-calbindin antibody (red) to highlight Purkinje cells. For IF, Hoechst dye (blue) was used to mark nuclei. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-017-0838-1), licensed under a CC-BY license")