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RBCK1 Antibody

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Orthogonal Strategies: Western Blot: RBCK1 Antibody [NBP1-88301] - Analysis in human cell lines SK-MEL-30 and HeLa using anti-RBCK1 antibody. Corresponding RBCK1 RNA-seq data are presented for the same cell ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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RBCK1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SVEDAQMHTVTIWLTVRPDMTVASLKDMVFLDYGFPPVLQQWVIGQRLARDQETLHSHGVRQNGDSAYLYLLSARNTSLNPQELQRERQLRMLEDLGFKDLTLQPRGPLEPGPPKPGVPQEPGRGQPDAVPEP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RBCK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04 - 0.4 ug/ml
Control Peptide
RBCK1 Protein (NBP1-88301PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-88301 in the following application:

Publications
Read Publications using
NBP1-88301 in the following applications:

  • IB
    1 publication
  • IP
    1 publication
  • WB
    7 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30209176). Expected reactivity in Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RBCK1 Antibody

  • C20orf18
  • EC 6.3.2.-
  • HBV associated factor 4
  • HBV-associated factor 4
  • Heme-oxidized IRP2 ubiquitin ligase 1
  • Hepatitis B virus X-associated protein 4
  • HOIL-1
  • RanBP-type and C3HC4-type zinc finger containing 1
  • ranBP-type and C3HC4-type zinc finger-containing protein 1
  • RBCC protein interacting with PKC1
  • RBCK2
  • RNF54RING finger protein 54
  • UBCE7IP3HOIL1
  • ubiquitin conjugating enzyme 7 interacting protein 3
  • Ubiquitin-conjugating enzyme 7-interacting protein 3
  • XAP3
  • XAP4chromosome 20 open reading frame 18
  • ZRANB4

Background

RBCK1 is encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RBCK1 Antibody (NBP1-88301)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IB, IP, WB.


Filter By Application
IB
(1)
IP
(1)
WB
(7)
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Human
(7)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP1-88301 Applications Species
Kouki Hoshino, Seshiru Nakazawa, Takehiko Yokobori, Kei Hagiwara, Norihiro Ishii, Mariko Tsukagoshi, Takamichi Igarashi, Kenichiro Araki, Norifumi Harimoto, Fuminori Tokunaga, Ken Shirabe RNF31 promotes proliferation and invasion of hepatocellular carcinoma via nuclear factor kappaB activation Scientific Reports 2024-01-03 [PMID: 38172174]
Peng R, Wang CK, Wang-Kan X et al. Human ZBP1 induces cell death-independent inflammatory signaling via RIPK3 and RIPK1 EMBO reports 2022-10-21 [PMID: 36268590] (WB, Human) WB Human
Douglas T, Saleh M Cross-regulation between LUBAC and caspase-1 modulates cell death and inflammation J. Biol. Chem. 2020-03-02 [PMID: 32122970] (IP) IP
Yamanaka S, Sato Y, Oikawa D et al. Subquinocin, a small molecule inhibitor of CYLD and USP-family deubiquitinating enzymes, promotes NF-kappa B signaling Biochem. Biophys. Res. Commun. 2019-12-30 [PMID: 31898971]
RB Damgaard, PR Elliott, KN Swatek, ER Maher, P Stepensky, O Elpeleg, D Komander, Y Berkun OTULIN deficiency in ORAS causes cell type-specific LUBAC degradation, dysregulated TNF signalling and cell death EMBO Mol Med, 2019-03-01;11(3):. 2019-03-01 [PMID: 30804083]
MacDuff DA, Baldridge MT, Qaqish AM et al. HOIL1 is essential for the induction of type I and III interferons by MDA5 and regulates persistent murine norovirus infection. J. Virol. 2018-09-12 [PMID: 30209176] (WB, Mouse) WB Mouse
Polajnar M, Dietz MS, Heilemann M, Behrends C. Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella EMBO Rep. 2017-08-06 [PMID: 28784601] (Human) Human
Shibata Y, Tokunaga F, Goto E et al. HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains PLoS Pathog 2017-01-01 [PMID: 28103322] (IB, Human) IB Human
Nakazawa S, Oikawa D, Ishii R et al. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun. 2016-08-24 [PMID: 27552911] (WB) WB
Klein T, Fung SY, Renner F et al. The paracaspase MALT1 cleaves HOIL1 reducing linear ubiquitination by LUBAC to dampen lymphocyte NF-kappa signalling. Nat Commun 2015-11-03 [PMID: 26525107] (WB, Human) WB Human
Show All 13 Publications.

Review for RBCK1 Antibody (NBP1-88301) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-88301:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Mouse 04/28/2017
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Summary

ApplicationWestern Blot
Sample TestedBone marrow-derived dendritic cells
SpeciesMouse

Comments

Comments5% NFDM in TBS-T was used for blocking, primary and secondary antibody incubations.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RBCK1 Antibody (NBP1-88301) (0)

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Secondary Antibodies

 

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Recent Reviews

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Verified Customer
04/28/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol RBCK1