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Rat IL-6 Quantikine ELISA Kit

 (181 Publications)
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Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Conjugate
HRP

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Catalog# & Conjugate Size Price
HRP
SR6000B
HRP
PR6000B
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Rat IL-6 Quantikine ELISA Kit Summary

Background
The Quantikine Rat IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-6 in cell culture supernates, rat serum, and plasma. It contains E. coli-expressed recombinant rat IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant protein. Results obtained using natural rat IL-6 showed dos...e-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-6.
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Specificity
Natural and recombinant rat IL-6
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il6

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
R6000B in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IL-6 Quantikine ELISA Kit

  • B-cell differentiation factor
  • B-cell stimulatory factor 2
  • BSF2
  • BSF-2
  • CDF
  • CTL differentiation factor
  • HSF
  • hybridoma growth factor
  • IFNB2
  • IFN-beta-2
  • IL6
  • IL-6
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

Interleukin 6 (IL-6) is a multifunctional cytokine that plays important roles in host defense, acute phase reactions, immune responses, nerve cell functions and hematopoiesis (1-5). It is expressed by a variety of normal and transformed lymphoid and non-lymphoid cells. The production of IL-6 is up-regulated by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses. IL-4, IL-10 and IL-13 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections, trauma, autoimmune diseases, inflammations and malignancies (1-5). 
IL-6 is a prototypic member of the IL-6 superfamily of cytokines that share gp130 as a component required for signal transduction (4). The rat, mouse and human IL-6 cDNAs have been cloned (6-9). Rat IL-6 cDNA encodes a 211 amino acid (aa) residue precursor polypeptide with a hydrophobic signal peptide that is cleaved to generate the 187 aa residue mature protein. At the protein sequence level, there is approximately 39% identity between rat and human, and 87% identity between mouse and rat IL-6 (6). Although human and mouse IL-6 are equally active on mouse cells, mouse IL-6 is not active on human cells (9). 
The high-affinity IL-6 receptor complex, which mediates IL-6 bioactivity, consists of two membrane glycoproteins: an 80 kDa low-affinity IL-6-binding receptor (IL-6 R) and a 130 kDa signal-transducing protein (gp130) that lacks IL-6 binding ability (4). The binding of IL-6 to IL-6 R recruits gp130 to form a trimeric complex that dimerizes to form a hexameric complex, which transduces IL-6 signal (4). Soluble forms of both IL-6 R and gp130 have been detected in blood (11, 12). Soluble IL-6 R is capable of binding IL-6 and inducing homodimerization of membrane gp130 and subsequent signal transduction.

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Rat IL-6
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R6000B
Rat IL-6 Quantikine ELISA Kit
Species: Rt
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-6 (R6000B)(181)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: ELISA (Standard).


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ELISA (Standard)
(1)
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Human
(2)
Mouse
(6)
Rat
(174)
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Showing Publications 1 - 10 of 181. Show All 181 Publications.
Publications using R6000B Applications Species
Hassan, HM;Abdeen, AM;Abdelrahman, IY;Abdo, W;Mohammed, SS;Abdeen, A;Abdelkader, A;Olga, R;Fericean, L;Ibrahim, SF;Ghamry, HI;Elgendy, FS;Sorour, SM;Eldeeb, AA;Ahmed, O;Rashed, F;Bikheet, MM; Radioprotective potential of whey protein against gamma irradiation-induced lingual damage Frontiers in pharmacology 2023-12-13 [PMID: 38155907] (Rat) Rat
Deng, J;Golub, LM;Lee, HM;Bhatt, HD;Johnson, F;Xu, TM;Gu, Y; A novel modified-curcumin 2.24 resolves inflammation by promoting M2 macrophage polarization Scientific reports 2023-09-19 [PMID: 37726411] (Rat) Rat
Duan, R;Hou, J;Wang, X;Huang, Z;Cao, H;Hu, J;Peng, Q;Duan, H;Wang, Q;Chen, X; Prevotella histicola Transplantation Ameliorates Cognitive Impairment and Decreases Oxidative Stress in Vascular Dementia Rats Brain sciences 2023-07-29 [PMID: 37626492] (Rat) Rat
Alsuliam, SM;Albadr, NA;Alshammari, GM;Almaiman, SA;ElGasim Ahmed Yagoub, A;Saleh, A;Abdo Yahya, M; Lepidium sativum alleviates diabetic nephropathy in a rat model by attenuating glucose levels, oxidative stress, and inflammation with concomitant suppression of TGF-?1 Saudi journal of biological sciences 2023-08-01 [PMID: 37576066] (Rat) Rat
McColl, ER;Henderson, JT;Piquette-Miller, M; Dysregulation of Amino Acid Transporters in a Rat Model of TLR7-Mediated Maternal Immune Activation Pharmaceutics 2023-07-01 [PMID: 37514044] (Rat) Rat
Kim, HR;Lee, SH;Noh, EM;Choi, B;Seo, HY;Jang, H;Kim, SY;Park, MH; Therapeutic Effect of Enzymatically Hydrolyzed Cervi Cornu Collagen NP-2007 and Potential for Application in Osteoarthritis Treatment International journal of molecular sciences 2023-07-19 [PMID: 37511425] (Rat) Rat
Bai, Y;Mi, W;Meng, X;Dong, B;Jiang, Y;Lu, Y;Yu, Y; Hydrogen alleviated cognitive impairment and blood‒brain barrier damage in sepsis-associated encephalopathy by regulating ABC efflux transporters in a PPAR alpha -dependent manner BMC neuroscience 2023-07-20 [PMID: 37474902] (Mouse) Mouse
Elwany, NE;Abdelhamid, AM;Mohamed, NM;Khalil, SS;Elsayed Orabi, EE;Abdelfattah, AM; Vinpocetine alleviates intestinal ischemia/reperfusion injury and enhances M2 macrophage polarization in rats: Role of SIRT1/SOCS3/STAT3 signaling pathway International immunopharmacology 2023-07-15 [PMID: 37459783] (Rat) Rat
Clarysse, M;Accarie, A;Panisello-Roselló, A;Farré, R;Canovai, E;Monbaliu, D;De Hertogh, G;Vanuytsel, T;Pirenne, J;Ceulemans, LJ; Intravenous Polyethylene Glycol Alleviates Intestinal Ischemia-Reperfusion Injury in a Rodent Model International journal of molecular sciences 2023-06-28 [PMID: 37445954] (Rat) Rat
Queiroz, JLC;Medeiros, I;Lima, MSR;Carvalho, FMC;Camillo, CS;Santos, PPA;Guerra, GCB;da Silva, VC;Schroeder, HT;Krause, M;Morais, AHA;Passos, TS; Efficacy of Carotenoid-Loaded Gelatin Nanoparticles in Reducing Plasma Cytokines and Adipocyte Hypertrophy in Wistar Rats International journal of molecular sciences 2023-06-26 [PMID: 37445834] (Rat) Rat
Show All 181 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-6 (R6000B). (Showing 1 - 1 of 1 FAQs).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.
Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.
The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.
The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.
VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.
TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.
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Bioinformatics

Gene Symbol Il6