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RASSF1 Antibody (OTI2B11) [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2B11
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73786AF350
Alexa Fluor 405
NBP2-73786AF405
Alexa Fluor 488
NBP2-73786AF488
Alexa Fluor 532
NBP2-73786AF532
Alexa Fluor 594
NBP2-73786AF594
Alexa Fluor 647
NBP2-73786AF647
Alexa Fluor 700
NBP2-73786AF700
Alexa Fluor 750
NBP2-73786AF750
Allophycocyanin
NBP2-73786APC
Biotin
NBP2-73786B
CoraFluor 1
NBP2-73786CL1
DyLight 350
NBP2-73786UV
DyLight 405
NBP2-73786V
DyLight 488
NBP2-73786G
DyLight 550
NBP2-73786R
DyLight 594
NBP2-73786DL594
DyLight 680
NBP2-73786FR
DyLight 755
NBP2-73786IR
FITC
NBP2-73786F
HRP
NBP2-73786H
Janelia Fluor 525
NBP2-73786JF525
Janelia Fluor 549
NBP2-73786JF549
Janelia Fluor 585
NBP2-73786JF585
Janelia Fluor 635
NBP2-73786JF635
Janelia Fluor 646
NBP2-73786JF646
Janelia Fluor 669
NBP2-73786JF669
PE
NBP2-73786PE
PerCP
NBP2-73786PCP
Unconjugated
NBP2-03644
mFluor Violet 450 SE
NBP2-73786MFV450
mFluor Violet 500 SE
NBP2-73786MFV500
mFluor Violet 610 SE
NBP2-73786MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-03644
Azide and BSA Free
NBP2-73786
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RASSF1 Antibody (OTI2B11) [DyLight 650] Summary

Immunogen
Full length recombinant protein of human RASSF1 (NP_009113) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RASSF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for RASSF1 Antibody (OTI2B11) [DyLight 650]

  • 123F2
  • NORE2A
  • pancreas-specific ras association domain family 1 protein
  • Ras association (RalGDS/AF-6) domain family member 1
  • ras association domain-containing protein 1
  • RASSF1A
  • RDA32cardiac-specific ras association domain family 1 protein
  • REH3P21
  • tumor suppressor protein RDA32
  • WUGSC:H_LUCA12.5

Background

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability. Agathanggelou A. et al

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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RASSF1 Antibody
(OTI2B11) [DyLight 65
NBP2-73786C
RASSF1 Antibody (OTI2B11) [DyLight 650]
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RASSF1