![Western Blot: RASSF1 Antibody (3F3) [NBP1-04342] - Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: RASSF1A transfected 293T cell lysate](http://images.novusbio.com/fullsize/RASSF1-Antibody-3F3-Western-Blot-NBP1-04342-img0002.jpg "Western Blot: RASSF1 Antibody (3F3) [NBP1-04342] - Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: RASSF1A transfected 293T cell lysate")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 3F3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Recombinant human RASSF1 (1-340 aa), purified from E. coli (NP_009113). |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RASSF1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 39 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
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Secondary Antibodies |
Isotype Controls |
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Role of RASSF1A in Death Receptor-Dependent Apoptosis Death-receptor apoptosis, or cell death, is essential for cellular growth regulation; its disruption is expressed in a variety of cancers. We at Novus Biologicals are one of the leading antibody suppliersfor apoptosis and cancer research groups, and t... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (3F3) [NBP1-04342] - Analysis of RASSF1A in A431 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/RASSF1-Antibody-3F3-Immunocytochemistry-Immunofluorescence-NBP1-04342-img0004.jpg "Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (3F3) [NBP1-04342] - Analysis of RASSF1A in A431 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).")
![Western Blot: RASSF1 Antibody (3F3) [NBP1-04342] - Cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysate](http://images.novusbio.com/fullsize/RASSF1-Antibody-3F3-Western-Blot-NBP1-04342-img0001.jpg "Western Blot: RASSF1 Antibody (3F3) [NBP1-04342] - Cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysate")
![Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (3F3) [NBP1-04342] - Analysis of RASSF1A in HeLa cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/RASSF1-Antibody-3F3-Immunocytochemistry-Immunofluorescence-NBP1-04342-img0003.jpg "Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (3F3) [NBP1-04342] - Analysis of RASSF1A in HeLa cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")