![Western Blot: RanBP1 Antibody [NB100-1443] - Staining of A431 (A) with antibody at 0.03 ug/mL, HeLa (B) with antibody at 0.5 ug/mL and MCF7 (C) cell lysate with antibody at 0.1 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Western-Blot-NB100-1443-img0002.jpg "Western Blot: RanBP1 Antibody [NB100-1443] - Staining of A431 (A) with antibody at 0.03 ug/mL, HeLa (B) with antibody at 0.5 ug/mL and MCF7 (C) cell lysate with antibody at 0.1 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-LSVKEETKEDAEEKQ corresponding to C-Terminus according to NP_002873.1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | RANBP1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-1443 | Applications | Species |
|---|---|---|
| Bischoff FR, Krebber H, Smirnova E et al. Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1. EMBO J 1995-02-15 [PMID: 7882974] |
Secondary Antibodies |
Isotype Controls |
Research Areas for RanBP1 Antibody (NB100-1443)Find related products by research area.
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| Gene Symbol | RANBP1 |
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![Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NB100-1443] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/RanBP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1443-img0004.jpg "Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NB100-1443] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: RanBP1 Antibody [NB100-1443] - Staining of paraffin embedded Human Kidney with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Immunohistochemistry-Paraffin-NB100-1443-img0006.jpg "Immunohistochemistry-Paraffin: RanBP1 Antibody [NB100-1443] - Staining of paraffin embedded Human Kidney with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Flow Cytometry: RanBP1 Antibody [NB100-1443] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Flow-Cytometry-NB100-1443-img0005.jpg "Flow Cytometry: RanBP1 Antibody [NB100-1443] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: RanBP1 Antibody [NB100-1443] - Staining of Human Duodenum lysate with antibody at 0.3 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Western-Blot-NB100-1443-img0001.jpg "Western Blot: RanBP1 Antibody [NB100-1443] - Staining of Human Duodenum lysate with antibody at 0.3 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NB100-1443] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/RanBP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1443-img0003.jpg "Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NB100-1443] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
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