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Rac1 Antibody

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Immunocytochemistry/Immunofluorescence: Rac1 Antibody [NB100-82169] - Staining of methanol-fixed Hela cells using Rac1 (Ab-71) antibody
Immunocytochemistry/ Immunofluorescence: Rac1 Antibody [NB100-82169] - Staining of methanol-fixed Hela cells using Rac1 (Ab-71) antibody
Western Blot: Rac1 Antibody [NB100-82169] - Western blot analysis of extracts from various cells using NB100-82169

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Rac1 Antibody Summary

Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Rac1 around amino acids 69~73 (P-L-S-Y-P).
Specificity
Detects endogenous levels of total Rac1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry
  • Western Blot 1:500-1:1000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rac1 Antibody

  • Cell migration-inducing gene 5 protein
  • MGC111543
  • p21-Rac1
  • p21-Rac1migration-inducing gene 5
  • Rac1
  • Ras-like protein TC25
  • ras-related C3 botulinum toxin substrate 1 (rho family, small GTP bindingprotein Rac1)
  • ras-related C3 botulinum toxin substrate 1
  • rho family, small GTP binding protein Rac1
  • TC25
  • TC-25

Background

Rac1 encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. Aoki K, et al. (2005) Mol Biol Cell; 16(5): 2207

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-82169
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Species: Hu, Mu, Rt
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAC1
Entrez
Uniprot