![Western Blot: PUM2 Antibody [NBP2-20009] - Sample (20 ug of whole cell lysate) A: Human ESC 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NBP2-20009-img0001.jpg "Western Blot: PUM2 Antibody [NBP2-20009] - Sample (20 ug of whole cell lysate) A: Human ESC 5% SDS PAGE gel, diluted at 1:2000.")
| Reactivity | Hu, Mu, Rt, Ch, FiSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PUM2. The exact sequence is proprietary. |
| Localization | Cytoplasm |
| Predicted Species | Rat (100%), Chicken (100%), Fish (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PUM2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 114 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for PUM2 Antibody (NBP2-20009)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PUM2 Antibody [NBP2-20009] - Pumilio 2 antibody detects Pumilio 2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Pumilio 2 stained by Pumilio 2 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PUM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20009-img0003.jpg "Immunocytochemistry/Immunofluorescence: PUM2 Antibody [NBP2-20009] - Pumilio 2 antibody detects Pumilio 2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Pumilio 2 stained by Pumilio 2 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Western Blot: PUM2 Antibody [NBP2-20009] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Pumilio 2 antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NBP2-20009-img0002.jpg "Western Blot: PUM2 Antibody [NBP2-20009] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Pumilio 2 antibody [C2C3], C-term diluted at 1:500.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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