Home » PTK7/CCK4 » PTK7/CCK4 Antibodies » PTK7/CCK4 Antibody (525222)

PTK7/CCK4 Antibody (525222)

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody (Catalog # MAB4499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody ...read more
PTK7/CCK4 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody (Catalog # MAB4499) at 25 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTK7/CCK4 at approximately 168 kDa (as indicated) using 20 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clone
525222
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB4499U
Alexa Fluor 405
FAB4499V
Alexa Fluor 532
FAB4499X
Alexa Fluor 594
FAB4499T
Alexa Fluor 647
FAB4499R
Alexa Fluor 700
FAB4499N
Alexa Fluor 750
FAB4499S
Biotin
FAB4499B
CoraFluor 1
FAB4499CL1
DyLight 350
FAB4499D
DyLight 405
FAB4499E
DyLight 488
FAB4499K
DyLight 550
FAB4499L
DyLight 594
FAB4499M
DyLight 650
FAB4499W
DyLight 680
FAB4499Y
DyLight 755
FAB4499Z
FITC
FAB4499F
HRP
FAB4499H
Janelia Fluor 525
FAB4499JF525
Janelia Fluor 549
FAB4499I
Janelia Fluor 646
FAB4499J
Janelia Fluor 669
FAB4499JF669
PE
FAB4499P
PerCP
FAB4499C
mFluor Violet 450 SE
FAB4499MFV450
mFluor Violet 500 SE
FAB4499MFV500
mFluor Violet 610 SE
FAB4499MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4499-SP
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PTK7/CCK4 Antibody (525222) Summary

Immunogen
E. coli-derived recombinant human PTK7/CCK4
Ala31-Ser199
Accession # Q13308
Specificity
Detects human and rat PTK7/CCK4 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTK7
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
MAB4499 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTK7/CCK4 Antibody (525222)

  • CCK4
  • CCK-4
  • CCK4Colon carcinoma kinase 4
  • colon carcinoma kinase-4
  • Protein-tyrosine kinase 7
  • Pseudo tyrosine kinase receptor 7
  • PTK7 protein tyrosine kinase 7
  • PTK7
  • Tyrosine-protein kinase-like 7

Background

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is a receptor tyrosine kinase (RTK) involved in colon carcinoma development and/or proliferation. Similar to the RTK HER3, PTK7 contains a catalytically inactive tyrosine kinase domain, suggesting a potential tumor-characteristic role as a signal amplifier or modulator for an as yet unidentified kinase-competent partner. PTK7 may also play a role during gastrulation in the rodent embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PTK7/CCK4 Antibody (MAB4499)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, Western Blot.


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(1)
Western Blot
(1)
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Bioinformatics

Gene Symbol PTK7
Uniprot