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PRPK Antibody

Images

 
Western Blot: PRPK Antibody [NBP1-89919] - Analysis in control (vector only transfected HEK293T lysate) and TP53RK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: PRPK Antibody [NBP1-89919] - Staining of human gallbladder shows strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PRPK Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AAVIKHRFPKGYRHPALEARLGRRRTVQEARALLRCRRAGISAPVVFFVDYASNCLYMEEIEGSVTVRDYIQSTMETEKTPQGLSNLAKTIGQV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TP53RK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
PRPK Protein (NBP1-89919PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PRPK Antibody

  • BUD32
  • C20orf64
  • chromosome 20 open reading frame 64
  • dJ101A2
  • dJ101A2.2
  • EC 2.7.11.1
  • Nori-2
  • Nori-2p
  • p53-related protein kinase
  • prpk
  • TP53 regulating kinase
  • TP53-regulating kinase

Background

Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in itsactivation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TP53RK