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Proteasome 20S alpha 6 Antibody

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Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538] - A: 293T B: A431 C: H1299 D: Hela 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538] - A: 293T B: A431 C: H1299 D: Hela 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 6 Antibody [NBP1-33538] - Paraformaldehyde-fixed A431, using Proteasome 20S alpha 6 (NBP1-33538) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538] - Paraffin-embedded mouse liver. Proteasome 20S alpha 6 antibody dilution: 1:500.
Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGEProteasome 20S alpha 6 antibody dilution: 1:1000
Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538] - A. 50 ug rat liver lysate/extract 12 % SDS-PAGE Proteasome 20S alpha 6 antibody dilution: 1:1000
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538] - Paraffin-embedded mouse lung. Proteasome 20S alpha 6 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538] - Paraffin-embedded mouse urinary bladder. Proteasome 20S alpha 6 antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Pm, RM, ShSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Proteasome 20S alpha 6 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 6. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Predicted Species
Porcine (98%), Chimpanzee (100%), Bovine (100%), Rhesus Macaque (100%), Chicken (96%), Sheep (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMA6
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Proteasome 20S alpha 6 Antibody

  • EC 3.4.25.1
  • IOTA
  • Macropain iota chain
  • macropain subunit iota
  • MGC22756
  • MGC2333
  • MGC23846
  • Multicatalytic endopeptidase complex iota chain
  • p27K
  • p27KPROS-27
  • PROS27
  • PROS-27
  • PROS2727 kDa prosomal protein
  • prosomal P27K protein
  • proteasome (prosome, macropain) subunit, alpha type, 6
  • Proteasome 20S alpha 6
  • Proteasome iota chain
  • proteasome subunit alpha type-6
  • proteasome subunit iota
  • PSMA6

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol PSMA6