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Galectin-2 Antibody

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Western blot shows lysates of mouse stomach tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Galectin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6667) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Galectin-2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse stomach tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Galectin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6667) followed by HRP-conjugated Anti-Sheep IgG ...read more
Galectin-2 was detected in perfusion fixed frozen sections of mouse intestine using Mouse Galectin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6667) at 5 µg/mL overnight at 4 °C. Tissue was stained ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF6667-SP
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Galectin-2 Antibody Summary

Immunogen
E. coli-derived recombinant mouse Galectin-2
Ser2-Glu130 (Gly36Val)
Accession # Q9CQW5
Specificity
Detects mouse Galectin-2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human Galectin-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
LGALS2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-2 Antibody

  • Beta-galactoside-binding lectin L-14-II
  • GAL2
  • Gal-2
  • galectin 2
  • Galectin2
  • Galectin-2
  • HL14gal-2
  • Lactose-binding lectin 2
  • lectin, galactoside-binding, soluble, 2
  • LGALS2
  • MGC75071
  • S-Lac lectin 2

Background

Galectin-2 (Gal-2; also LEG2, HL14 and L-14-II) is a 14-15 kDa member of the S-type (1-CRD type) lectin family of proteins. It is a potentially secreted, noncovalent homodimer that is expressed by stomach mucosal columnar epithelium, small intestine Goblet cells and keratinocytes. Galectin-2 is reported to bind to CD29, and initiate CD8+ T cell apoptosis. Structurally, it serves to bind nonsialylated poly-NAcetyllactosamine motifs. Mouse Galectin-2 is 130 amino acids (aa) in length. There is one galectin domain (aa 4-130) that contains one dimerization interface (aa 123-128). Full-length mouse Galectin-2 shares 86% and 66% aa identity with rat and human Galectin-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LGALS2
Uniprot