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PRAF1 Antibody

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Immunocytochemistry/ Immunofluorescence: PRAF1 Antibody [NBP2-56173] - Staining of human cell line HeLa shows localization to nucleus & nucleoli fibrillar center.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PRAF1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LPPCYDDAAKPEDVYKFEDLLSPAEYEALQSPSEAFRNVTSEEILKMIEENSHCTFVIEALKSLPSDVESRDRQARCIWFLDTLIKFRAHRVVKRKSALGP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR1E
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PRAF1 Recombinant Protein Antigen (NBP2-56173PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PRAF1 Antibody

  • DNA-directed RNA polymerase I subunit E
  • DNA-directed RNA polymerase I subunit RPA49
  • FLJ13390
  • FLJ13970
  • FLJ43482
  • PAF53RNA polymerase I-associated factor 1
  • polymerase (RNA) I associated factor 1
  • polymerase (RNA) I polypeptide E, 53kDa
  • PRAF1
  • RNA polymerase I associated factor 53
  • RNA polymerase I subunit A49
  • RNA polymerase I-associated factor 53
  • RP11-405L18.3

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleosidetriphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to beinvolved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I andUBTF/UBF

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol POLR1E