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POR/Cytochrome P450 Reductase Antibody (739613)

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human POR/Cytochrome P450 Reductase Monoclonal Antibody (Catalog # MAB6340) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for POR/Cytochrome P450 Reductase at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human POR/Cytochrome P450 Reductase Monoclonal Antibody (Catalog # MAB6340) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
739613
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB6340-SP
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POR/Cytochrome P450 Reductase Antibody (739613) Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human POR/Cytochrome P450 Reductase
Arg45-Ser677
Accession # P16435
Specificity
Detects human POR/Cytochrome P450 Reductase in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
POR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for POR/Cytochrome P450 Reductase Antibody (739613)

  • CPR
  • CYPOR
  • CYPORFLJ26468
  • Cytochrome P450 Reductase
  • DKFZp686G04235
  • EC 1.6.2.4
  • NADPH--cytochrome P450 reductase
  • NADPH-dependent cytochrome P450 reductase
  • P450 (cytochrome) oxidoreductase
  • P450R
  • POR

Background

NADPH-Cytochrome P450 Reductase (POR, P450R) is an essential component of the cytochrome P450 monooxygenase system of eukaryotic cells (1). POR is anchored in the endoplasmic reticulum membrane with its catalytic domain residing in the cytosol. POR is a flavoprotein, containing one molecule each of FMN and FAD, which are essential for the transfer of electrons from NADPH to the cytochromes P450 (2). This reduction is necessary for cytochromes P450 to perform each cycle of oxidation. POR is also capable of transferring electrons to cytochrome b5, heme oxygenase, the fatty acid elongation system, and other proteins. Mutations of POR can result in disordered steroidogenesis and Antley-Bixler syndrome.

  1. Philips, A.H. and R.G. Langdon (1962) J. Biol. Chem. 237:2652.
  2. Iyanagi, T. and H.S. Mason (1973) Biochemistry 12:2291.
  3. Flueck C.E. et al. (2004) Nat. Genet. 36:228.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol POR
Uniprot