PNUTS/PPP1R10 Antibody

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Western Blot: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of human PNUTS by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity ...read more
Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of human PNUTS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PNUTS (NB100-604). ...read more
Western Blot: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of Human PNUTS on HeLa whole cell lysates using NB100-604. IP of PNUTS was successfully performed with NB100-603, NB100-605, and another PNUTS polyclonal. ...read more
Biological Strategies: Western Blot: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of Human PNUTS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity ...read more
Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PNUTS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PNUTS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Examples of PNUTS staining: (a) comparison of PNUTS/PPP1R10 staining in (1) normal and (2) cancerous prostate glands of the same TMA spot. Cancer spots with (b) ...read more
Independent Antibodies: Immunoprecipitation: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of human PNUTS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP ...read more
Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Examples of PNUTS staining: (a) comparison of PNUTS staining in (1) normal & (2) cancerous prostate glands of the same TMA spot. Cancer spots with (b) lack of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PNUTS/PPP1R10 Antibody Summary

Immunogen
A synthetic peptide, mapping to a region region between residues 350 and 400 of human Phosphatase Nuclear Targeting Subunit (a.k.a. Protein Phosphatase 1, regulatory subunit 10) using the numbering given in TrEMBL entry Q96QC0 (GeneID 5514).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP1R10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500- 1:2000
  • Immunoprecipitation 1-10 ug/mg lysate
  • Western Blot 1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publications using
NB100-604 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PNUTS/PPP1R10 Antibody

  • CAT53
  • FB19PP1-binding protein of 114 kDa
  • MHC class I region proline-rich protein CAT53
  • p99
  • Phosphatase 1 nuclear targeting subunit
  • phosphatase nuclear targeting subunit
  • PNUTS
  • PNUTSPP1R10
  • PPP1R10
  • Protein FB19
  • protein phosphatase 1 regulatory subunit 10
  • protein phosphatase 1, regulatory (inhibitor) subunit 10
  • protein phosphatase 1, regulatory subunit 10
  • serine/threonine-protein phosphatase 1 regulatory subunit 10

Background

Protein phosphatase 1 (PP1) is found in the cell nucleus and has been implicated in several aspects of nuclear function. Phosphatase 1 nuclear targeting subunit (PNUTS) is a novel protein that exhibits a discreet nuclear compartmentalization and is colocalized with chromatin at distinct phases during mitosis. The subcellular localization of PP1 and the activity toward substrates involved in many aspects of cell physiology have previously been shown to be regulated by association with noncatalytic targeting subunits. The properties of PNUTS are consistent with its role as a targeting subunit for the regulation of nuclear PP1 function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PNUTS/PPP1R10 Antibody (NB100-604)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Bioinformatics

Gene Symbol PPP1R10
Entrez
Uniprot