![Western Blot: Plexin D1 Antibody [NBP1-33634] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Plexin D1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Plexin-D1-Antibody-Western-Blot-NBP1-33634-img0006.jpg "Western Blot: Plexin D1 Antibody [NBP1-33634] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Plexin D1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Plexin D1. The exact sequence is proprietary. |
| Localization | Cell membrane, Single-pass membrane protein |
| Predicted Species | Rat (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PLXND1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 212 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Immunohistochemistry_21496.jpg)
![Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Immunohistochemistry_21497.jpg)
![Western Blot Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Western_Blot_21557.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IHC-Fr | Mouse | 06/23/2021 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Plexin D1 Antibody [NBP1-33634] - Analysis of methanol-fixed HeLa, using Plexin D1antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Plexin-D1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33634-img0004.jpg "Immunocytochemistry/Immunofluorescence: Plexin D1 Antibody [NBP1-33634] - Analysis of methanol-fixed HeLa, using Plexin D1antibody at 1:500 dilution.")
![Immunohistochemistry-Frozen: Plexin D1 Antibody [NBP1-33634] - PlexinD1 on an E12.5 mouse Left Ventricle. Antibody at 1:100. Alexa Fluor 488 secondary antibody at 1:1000. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Plexin-D1-Antibody-Immunohistochemistry-Frozen-NBP1-33634-img0007.jpg "Immunohistochemistry-Frozen: Plexin D1 Antibody [NBP1-33634] - PlexinD1 on an E12.5 mouse Left Ventricle. Antibody at 1:100. Alexa Fluor 488 secondary antibody at 1:1000. IHC-Fr image submitted by a verified customer review.")
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![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Immunohistochemistry Neuropilin-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin2_AF567_Immunohistochemistry_16084.jpg)
![Western Blot Neuropilin-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin2_AF567_Western_Blot_20048.jpg)
![Simple Western Neuropilin-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin2_AF567_Simple_Western_20049.jpg)
![SDS-PAGE ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/ErbB2_1129-ER_70.jpg)
![Bioactivity ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/1129-er_recombinant-human-erbb2-her2-fc-chimera-protein-cf-bioactivity-7122020142841.jpg)
![Immunocytochemistry Plexin A2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A2_AF5486_Immunocytochemistry_11380.jpg)
![Immunocytochemistry Plexin A2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A2_AF5486_Immunocytochemistry_11381.jpg)
![Simple Western Plexin A2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A2_AF5486_Simple_Western_17021.jpg)
![Western Blot Plexin B2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_B2_AF5329_Western_Blot_5686.jpg)
![Immunohistochemistry Plexin B2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_B2_AF5329_Immunohistochemistry_7294.jpg)
![Immunohistochemistry Plexin B2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_B2_AF5329_Immunohistochemistry_7295.jpg)
![Bioactivity Semaphorin 3A [Unconjugated]](https://images.novusbio.com/images2/Semaphorin_3A_1250S3_1473.jpg)
![SDS-PAGE CXCL12/SDF-1 alpha [Unconjugated]](https://images.novusbio.com/images2/CXCL12_SDF-1_alpha_350-NS_308.jpg)
![Bioactivity CXCL12/SDF-1 alpha [Unconjugated]](https://images.novusbio.com/images2/CXCL12SDF1_alpha_350NS_1178.jpg)
![CCL19/MIP-3 beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "CCL19/MIP-3 beta [Unconjugated]")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
