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PKR Antibody (1D11)

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Western Blot: PKR Antibody (1D11) [H00005610-M02] - Analysis of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M02), clone 1D11.Lane 1: EIF2AK2 transfected lysate(62.1 KDa).Lane 2: Non-transfected lysate.
Western Blot: PKR Antibody (1D11) [H00005610-M02] - Analysis of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M02), clone 1D11.Lane 1: EIF2AK2 transfected lysate(62.1 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: PKR Antibody (1D11) [H00005610-M02] - Analysis of monoclonal antibody to EIF2AK2 on HeLa cell. Antibody concentration 30 ug/ml.
Immunohistochemistry-Paraffin: PKR Antibody (1D11) [H00005610-M02] - Analysis of monoclonal antibody to EIF2AK2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 1.5 ug/ml.
Western Blot: PKR Antibody (1D11) [H00005610-M02] - EIF2AK2 monoclonal antibody (M02), clone 1D11 Analysis of EIF2AK2 expression in K-562.
ELISA: PKR Antibody (1D11) [H00005610-M02] - Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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PKR Antibody (1D11) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
EIF2AK2 (NP_002750, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGN
Specificity
EIF2AK2 (1D11)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EIF2AK2
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Publications
Read Publications using H00005610-M02.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PKR Antibody (1D11)

  • double stranded RNA activated protein kinase
  • EC 2.7.11.1
  • eIF-2A protein kinase 2
  • EIF2AK1
  • EIF2AK2
  • eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase
  • interferon-induced, double-stranded RNA-activated protein kinase
  • interferon-inducible double stranded RNA dependent
  • interferon-inducible elF2alpha kinase
  • Interferon-inducible RNA-dependent protein kinase
  • P68 Kinase
  • PKR
  • PKRp68 kinase
  • PPP1R83
  • PRKR
  • PRKRMGC126524
  • Protein Kinase R
  • Protein kinase RNA-activated

Background

Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PKR Antibody (1D11)
H00005610-M02
PKR Antibody (1D11)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC

Publications for PKR Antibody (H00005610-M02)(3)

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol EIF2AK2
Entrez
OMIM
Uniprot