PKC epsilon Antibody (OTI4G8) [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI4G8
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Order Details

PKC epsilon Antibody (OTI4G8) [DyLight 405] Summary

Immunogen
Full length recombinant protein of human PKC epsilon (NP_005391) produced in HEK293T cells.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PRKCE
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for PKC epsilon Antibody (OTI4G8) [DyLight 405]

  • EC 2.7.11
  • EC 2.7.11.13
  • MGC125656
  • nPKC-epsilon
  • PKC epsilon
  • PKCE
  • PKCEMGC125657
  • PRKCE
  • protein kinase C epsilon type
  • protein kinase C, epsilon

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKC epsilon Antibody (NBP2-73438V) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRKCE