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PIST Antibody

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Western Blot: PIST Antibody [NB100-1375] - Analysis of PIST/FIG/GOPC on Human Frontal Cortex lysate using this antibody at 0.05 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity Hu, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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PIST Antibody Summary

Immunogen
Peptide with sequence C-LDDLHTLYHKKSY corresponding to C-Terminus according to NP_065132.1, NP_001017408.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_065132.1; NP_001017408.1).
Predicted Species
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GOPC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.05 - 0.2 ug/ml
Application Notes
IF successful usage on HeLa cells. WB: Approx. 55 kDa band observed in human brain (frontal cortex) lysates (calculated MW of 50.5 kDa band according to NP_065132.1).
Control
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-1375.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIST Antibody

  • CALPDZ protein interacting specifically with TC10
  • CFTR-associated ligand
  • dJ94G16.2
  • FIGdJ94G16.2 PIST
  • Fused in glioblastoma
  • golgi-associated PDZ and coiled-coil motif containing
  • Golgi-associated PDZ and coiled-coil motif-containing protein
  • GOPC1
  • PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10
  • PISTGolgi associated PDZ and coiled-coil motif containing protein

Background

GenBank Accession Number - NP_065132. PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST is a PDZ domain-containing Golgi protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIST Antibody (NB100-1375)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GOPC
Entrez
Uniprot