Pirin Antibody

Images

 
Western Blot: Pirin Antibody [NBP2-19840] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGE Pirin antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: Pirin Antibody [NBP2-19840] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Pirin Antibody [NBP2-19840] - Paraffin-embedded mouse liver. Pirin antibody dilution: 1:500.
Western Blot: Pirin Antibody [NBP2-19840] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Pirin Antibody [NBP2-19840] - Immunohistochemical analysis of paraffin-embedded MPG xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Pirin Antibody [NBP2-19840] - Paraffin-embedded rat brain stem. Pirin antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Pirin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Pirin. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Bovine (90%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIR
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Pirin Antibody

  • EC 1.13.11.24
  • pirin (iron-binding nuclear protein)
  • pirin
  • Probable quercetin 2,3-dioxygenase PIR
  • Probable quercetinase

Background

This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pirin Antibody (NBP2-19840) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Pirin Products

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Calculators

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Bioinformatics

Gene Symbol PIR
Uniprot