![Western Blot: PIP5K1 alpha Antibody [NBP2-19833] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PIP5K1-alpha-Antibody-Western-Blot-NBP2-19833-img0005.jpg "Western Blot: PIP5K1 alpha Antibody [NBP2-19833] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Pm, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PIP5K1 alpha. The exact sequence is proprietary. |
| Localization | Cell membrane; Endomembrane system; Golgi apparatus; Golgi stack (By similarity) |
| Predicted Species | Rat (91%), Porcine (96%), Chimpanzee (99%), Bovine (94%), Rhesus Macaque (98%), Canine (95%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PIP5K1A |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 63 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for PIP5K1 alpha Antibody (NBP2-19833)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PIP5K1 alpha Antibody [NBP2-19833] - Immunofluorescence analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PIP5K1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19833-img0003.jpg "Immunocytochemistry/Immunofluorescence: PIP5K1 alpha Antibody [NBP2-19833] - Immunofluorescence analysis of methanol-fixed A549, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: PIP5K1 alpha Antibody [NBP2-19833] - CL1-0 xenograft, using PIPK I alpha antibody at 1:100 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PIP5K1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-19833-img0002.jpg "Immunohistochemistry-Paraffin: PIP5K1 alpha Antibody [NBP2-19833] - CL1-0 xenograft, using PIPK I alpha antibody at 1:100 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: PIP5K1 alpha Antibody [NBP2-19833] - Wild-type (WT) and PIP5K1A knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [N1N3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19833_rabbit-polyclonal-pip5k1-alpha-antibody-1610202419405721.jpg "Western Blot: PIP5K1 alpha Antibody [NBP2-19833] - Wild-type (WT) and PIP5K1A knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [N1N3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")