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PI 3-Kinase p110 gamma Antibody (OTI2B1)

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Western Blot: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PI 3-Kinase p110.
Western Blot: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PI 3-Kinase p110.
Immunohistochemistry: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of Human prostate. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of Adenocarcinoma of Human ovary tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI2B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73397AF350
Alexa Fluor 405
NBP2-73397AF405
Alexa Fluor 488
NBP2-73397AF488
Alexa Fluor 532
NBP2-73397AF532
Alexa Fluor 594
NBP2-73397AF594
Alexa Fluor 647
NBP2-73397AF647
Alexa Fluor 700
NBP2-73397AF700
Alexa Fluor 750
NBP2-73397AF750
Allophycocyanin
NBP2-73397APC
Biotin
NBP2-73397B
CoraFluor 1
NBP2-73397CL1
DyLight 350
NBP2-73397UV
DyLight 405
NBP2-73397V
DyLight 488
NBP2-73397G
DyLight 550
NBP2-73397R
DyLight 594
NBP2-73397DL594
DyLight 650
NBP2-73397C
DyLight 680
NBP2-73397FR
DyLight 755
NBP2-73397IR
FITC
NBP2-73397F
HRP
NBP2-73397H
Janelia Fluor 525
NBP2-73397JF525
Janelia Fluor 549
NBP2-73397JF549
Janelia Fluor 585
NBP2-73397JF585
Janelia Fluor 635
NBP2-73397JF635
Janelia Fluor 646
NBP2-73397JF646
Janelia Fluor 669
NBP2-73397JF669
PE
NBP2-73397PE
PerCP
NBP2-73397PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-73397
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PI 3-Kinase p110 gamma Antibody (OTI2B1) Summary

Immunogen
Full length human recombinant protein of human PIK3CG(NP_002640) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PIK3CG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
Theoretical MW
126.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PI 3-Kinase p110 gamma Antibody (OTI2B1)

  • 1-phosphatidylinositol 3-kinase
  • EC 2.7.1
  • EC 2.7.1.153
  • p110-gamma
  • p120-PI3K
  • phosphatidylinositol 3 kinase gamma, p110 gamma
  • phosphatidylinositol 3-kinase catalytic 110-kD gamma
  • phosphatidylinositol 3-kinase, catalytic, gamma polypeptide
  • Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma
  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
  • phosphoinositide-3-kinase gamma catalytic subunit
  • phosphoinositide-3-kinase, catalytic, gamma polypeptide
  • PI 3Kinase p110 gamma
  • PI 3-Kinase p110 gamma
  • PI3CG
  • PI3K
  • PI3K-gamma
  • PI3Kgamma
  • PI3K-gamma
  • PI3-kinase subunit gamma
  • PIK3
  • PIK3CG
  • PtdIns-3-kinase subunit gamma
  • PtdIns-3-kinase subunit p110-gamma

Background

PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p10. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K (1-2). Four isoforms of p110 has been found; alpha, beta, gamma, and the delta subunit (3). The gamma isoform is most clostly related to p110 alpha, and beta and has been shown not to interact with p85 in vivo (4). It has been found that in p110-gamma protein expression is lost in primary colorectal adeno-carcinomas and in colon cancer cell lines (5)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PIK3CG