Recombinant Human Phosphoserine phosphatase Protein Summary
Description |
An un-tagged recombinant protein corresponding to the amino acids 1-225 of Human Phosphoserine phosphatase Source: E.coli Amino Acid Sequence: MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AMGGAVPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKIIMIGDG ATDMEACPPA DAFIGFGGNV IRQQVKDNAK WYITDFVELL GELEE |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
PSPH |
Purity |
>95%, by SDS-PAGE |
Endotoxin Note |
< 1.0 EU per 1 microgram of protein (determined by LAL method) |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM HEPES buffer (pH 7.5), 1 mM DTT, 100 mM KCl |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
>95%, by SDS-PAGE |
Alternate Names for Recombinant Human Phosphoserine phosphatase Protein
Background
Human Phosphoserine phosphatase (hPSP) is an important enzyme in the phosphorylated pathway of serine biosynthesis, which contributes a major portion of the endogenous L-serine. Similar to known L-3-phosphoserine phosphatases, it catalyzed the Mg2+ General references: -dependent hydrolysis of L-phosphoserine and an exchange reaction between L-serine and L-phosphoserine. Recently, its complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the alpha-helical bundle domain, is important to substrate recognition and hydrolysis. Recombinant human hPSP was overexpressed in E. coli and purified by conventional chromatography.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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