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Phosphoserine phosphatase Antibody (3G12) - BSA Free

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Western Blot: Phosphoserine phosphatase Antibody (3G12) [NBP1-04297] - Cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-04297 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP. Lane 1: Jurkat cell lysate. Lane 2: K562 cell lysate. Lane 3: HepG2 cell lysate. Lane 4: A549 cell lysate. Lane 5: MCF7 cell lysate
Western Blot: Phosphoserine phosphatase Antibody (3G12) [NBP1-04297] - Cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-04297 (1:500). Proteins were visualized using a goat ...read more
Immunocytochemistry/ Immunofluorescence: Phosphoserine phosphatase Antibody (3G12) [NBP1-04297] - A431 cells stained with NBP1-04297 (1:100). Alexa Fluor 488 - conjugated secondary antibody was used (green). DAPI was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
3G12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-04297AF350
Alexa Fluor 405
NBP1-04297AF405
Alexa Fluor 488
NBP1-04297AF488
Alexa Fluor 532
NBP1-04297AF532
Alexa Fluor 594
NBP1-04297AF594
Alexa Fluor 647
NBP1-04297AF647
Alexa Fluor 700
NBP1-04297AF700
Alexa Fluor 750
NBP1-04297AF750
Biotin
NBP1-04297B
CoraFluor 1
NBP1-04297CL1
DyLight 350
NBP1-04297UV
DyLight 405
NBP1-04297V
DyLight 488
NBP1-04297G
DyLight 550
NBP1-04297R
DyLight 594
NBP1-04297DL594
DyLight 650
NBP1-04297C
DyLight 680
NBP1-04297FR
DyLight 755
NBP1-04297IR
FITC
NBP1-04297F
HRP
NBP1-04297H
Janelia Fluor 525
NBP1-04297JF525
Janelia Fluor 549
NBP1-04297JF549
Janelia Fluor 585
NBP1-04297JF585
Janelia Fluor 635
NBP1-04297JF635
Janelia Fluor 646
NBP1-04297JF646
Janelia Fluor 669
NBP1-04297JF669
mFluor Violet 450 SE
NBP1-04297MFV450
mFluor Violet 500 SE
NBP1-04297MFV500
mFluor Violet 610 SE
NBP1-04297MFV610
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Phosphoserine phosphatase Antibody (3G12) - BSA Free Summary

Immunogen
Recombinant human Phosphoserine phosphatase (1-225aa) purified from E. coli
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PSPH
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Phosphoserine phosphatase Antibody (3G12) - BSA Free

  • L-3-phosphoserine phosphatase
  • O-phosphoserine phosphohydrolase
  • phosphoserine phosphatase
  • PSPase
  • PSPEC 3.1.3.3

Background

Human phosphoserine phosphatase (HPSP), specific for D- and L- phosphoserine, has been identified in all human tissues. HPSP is a Mg(2+)-dependent phosphoserine phosphatase. The three dimensional structure of HPSP reveals the structural and functional role of the divalent cation in the active site of phosphatases. In particular, the complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the alpha-helical bundle domain, is important for the substrate recognition and hydrolysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Phosphoserine
phosphatase Antibody ...
NBP1-04297
Phosphoserine phosphatase Antibody (3G12) - BSA Free
Species: Hu
Applications: WB, ELISA, ICC/IF

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Bioinformatics

Gene Symbol PSPH
Uniprot