![Western Blot: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis using PLD2 mAb against human PLD2 (AA: 834-933) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-Western-Blot-NBP2-61784-img0007.jpg "Western Blot: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis using PLD2 mAb against human PLD2 (AA: 834-933) recombinant protein. (Expected MW is 37.4 kDa)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | 7E4D9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Phospholipase D2 (AA: 834-933) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PLD2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 106 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Phospholipase D2 Antibody (NBP2-61784)Find related products by research area.
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| Gene Symbol | PLD2 |
![Immunocytochemistry/Immunofluorescence: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of SK-OV-3 cells using PLD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-Immunocytochemistry-Immunofluorescence-NBP2-61784-img0004.jpg "Immunocytochemistry/Immunofluorescence: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of SK-OV-3 cells using PLD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.")
![Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of paraffin-embedded esophageal cancer tissues using PLD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-Immunohistochemistry-NBP2-61784-img0006.jpg "Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of paraffin-embedded esophageal cancer tissues using PLD2 mouse mAb with DAB staining.")
![Flow Cytometry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of Hela cells using PLD2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-ELISA-NBP2-61784-img0002.jpg "Flow Cytometry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of Hela cells using PLD2 mouse mAb (green) and negative control (red).")
![Western Blot: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis using PLD2 mAb against HEK293 (1) and PLD2 (AA: 834-933)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-Western-Blot-NBP2-61784-img0008.jpg "Western Blot: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis using PLD2 mAb against HEK293 (1) and PLD2 (AA: 834-933)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of MCF-7 cells using PLD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-Immunocytochemistry-Immunofluorescence-NBP2-61784-img0003.jpg "Immunocytochemistry/Immunofluorescence: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of MCF-7 cells using PLD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.")
![Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of paraffin-embedded renal tissues using PLD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-Immunohistochemistry-NBP2-61784-img0005.jpg "Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of paraffin-embedded renal tissues using PLD2 mouse mAb with DAB staining.")
![ELISA: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-ELISA-NBP2-61784-img0001.jpg "ELISA: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")