![Western Blot: Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody [NB300-635] - Lane 1: PDE4A Western blot positive Control (PDE4A5); Lane 2: Western blot with Phospho-PDE4A5](http://images.novusbio.com/fullsize/Phosphodiesterase-4A-PDE4A-[p-Ser686--p-Ser688]-Antibody-Western-Blot-NB300-635-img0001.jpg "Western Blot: Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody [NB300-635] - Lane 1: PDE4A Western blot positive Control (PDE4A5); Lane 2: Western blot with Phospho-PDE4A5")
| Reactivity | Hu, Mu, Rt, RbSpecies Glossary |
| Applications | WB, ELISA, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine. |
| Modification | p Ser686, p Ser688 |
| Localization | Cell Membrane |
| Specificity | This detects all known long-from variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phophorylation site. It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site. It does not cross-react with PDE4B, PDE4D or any other member of the PDE family. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PDE4A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Immunoprecipitation and Western Blot: Detects bands at 66 kDa (PDE4A1), 109 kDa (PDE4A5), 106 kDa(PDE4A8), 102 kDa (PDE4Ax) and 76 kDa (testis specific PDE4A variant). Optimal dilutions and concentrations should be determined by the end user. |
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody")
| Publication using NB300-635 | Applications | Species |
|---|---|---|
| Ale-Agha N, Goy C, Jakobs P et al. CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine PLoS Biol. 2018-06-01 [PMID: 29927970] (WB, Mouse) | WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for Phosphodiesterase 4A/PDE4A Antibody (NB300-635)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/IL-5_M5000_ELISA_237.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/DATA_IL5_M5000_ELISA_935.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")