![Western Blot: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4A.](http://images.novusbio.com/fullsize/Phosphodiesterase-4A-PDE4A-Antibody-1C8-Western-Blot-NBP2-02559-img0023.jpg "Western Blot: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4A.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI1C8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.92 mg/ml |
| Immunogen | Full length human recombinant protein of human PDE4A (NP_006193) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PDE4A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 72 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.92 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Phosphodiesterase 4A/PDE4A Antibody (NBP2-02559)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Immunofluorescent staining of HT29 cells using anti-PDE4A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphodiesterase-4A-PDE4A-Antibody-1C8-Immunocytochemistry-Immunofluorescence-NBP2-02559-img0013.jpg "Immunocytochemistry/Immunofluorescence: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Immunofluorescent staining of HT29 cells using anti-PDE4A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human prostate tissue using anti-PDE4A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphodiesterase-4A-PDE4A-Antibody-1C8-Immunohistochemistry-Paraffin-NBP2-02559-img0021.jpg "Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human prostate tissue using anti-PDE4A mouse monoclonal antibody.")
![Flow Cytometry: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE4A antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Phosphodiesterase-4A-PDE4A-Antibody-1C8-Flow-Cytometry-NBP2-02559-img0012.jpg "Flow Cytometry: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE4A antibody, and then analyzed by flow cytometry.")
![Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PDE4A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphodiesterase-4A-PDE4A-Antibody-1C8-Immunohistochemistry-Paraffin-NBP2-02559-img0014.jpg "Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PDE4A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDE4A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphodiesterase-4A-PDE4A-Antibody-1C8-Immunohistochemistry-Paraffin-NBP2-02559-img0015.jpg "Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDE4A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human colon tissue using anti-PDE4A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphodiesterase-4A-PDE4A-Antibody-1C8-Immunohistochemistry-Paraffin-NBP2-02559-img0016.jpg "Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human colon tissue using anti-PDE4A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human Kidney tissue using anti-PDE4A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphodiesterase-4A-PDE4A-Antibody-1C8-Immunohistochemistry-Paraffin-NBP2-02559-img0017.jpg "Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human Kidney tissue using anti-PDE4A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human liver tissue using anti-PDE4A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphodiesterase-4A-PDE4A-Antibody-1C8-Immunohistochemistry-Paraffin-NBP2-02559-img0018.jpg "Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human liver tissue using anti-PDE4A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human lung tissue using anti-PDE4A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphodiesterase-4A-PDE4A-Antibody-1C8-Immunohistochemistry-Paraffin-NBP2-02559-img0019.jpg "Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human lung tissue using anti-PDE4A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human pancreas tissue using anti-PDE4A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphodiesterase-4A-PDE4A-Antibody-1C8-Immunohistochemistry-Paraffin-NBP2-02559-img0020.jpg "Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human pancreas tissue using anti-PDE4A mouse monoclonal antibody.")
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![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
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![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/IL-5_M5000_ELISA_237.jpg)
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")