PGAP3 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

The L-type calcium channel is composed of four subunits: alpha-1, alpha-2, beta and gamma. The beta subunit of voltage-dependent calcium channels aids the function of the calcium channel by increasing the peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. CAB2 is a membrane protein that is expressed in all tissues. The unprocessed precursor is 660 amino acids in length and has a predicted molecular weight of 74.5KDa. It belongs to the calcium channel beta subunit family and contains one SH3 domain. CAB2 is involved in the MAPK signalling pathway and is also associated with Lambert-Eaton myasthenic syndrome.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBP2-05879

• PGAP3 Antibodies
• PGAP3 Lysate
• PGAP3 Protein