Novus Biologicals products are now on bio-techne.com

PGAM5 Antibody (CL0624)

Images

 
Western Blot: PGAM5 Antibody (CL0624) [NBP2-52947] - Lane 1: Marker [kDa] Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947] - Staining of human uterus shows moderate cytoplasmic positivity in glandular cells.
Genetic Strategies: Western Blot: PGAM5 Antibody (CL0624) [NBP2-52947] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PGAM5 antibody. Remaining relative ...read more
Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947] - Staining of human kidney shows either strong or moderate cytoplasmic immunoreactivity in subsets of renal tubules.
Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947] - Staining of human liver shows moderate cytoplasmic immunoreactivity in hepatocytes.
Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947] - Staining of human testis shows moderate to strong cytoplasmic positivity in the seminiferous ducts.
Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947] - Staining of human tonsil shows strong immunoreactivity in germinal center cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, KD
Clone
CL0624
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

PGAM5 Antibody (CL0624) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: NWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHVDGSLEKDRTLTPLGREQAELTGLRLASLGLKFNKIV
Epitope
NWDRREPLSLINVRK
Predicted Species
Mouse (94%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PGAM5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
PGAM5 Recombinant Protein Antigen (NBP2-52947PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for PGAM5 Antibody (CL0624)

  • Bcl-XL-binding protein v68
  • BXLBv68
  • EC 3.1.3.16
  • MGC5352
  • phosphoglycerate mutase family member 5BXLBV68
  • serine/threonine-protein phosphatase PGAM5, mitochondrial

Background

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate fora Keap1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-55288
Species: Fi, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP1-32822
Species: Al, Av, Hu, Mu, Pl, Rt, Ze
Applications: ChIP, Flow, ICC/IF, IHC, IHC-P, IP, KD, Simple Western, WB
H00007402-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP2-16148
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-76651
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-02477
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
MAB3024
Species: Hu, Mu, Rt
Applications: KO, Simple Western, WB
NBP2-66953
Species: Hu, Mu
Applications: IHC, IHC-Fr, IHC-P, WB
BC100-494
Species: Hu, Mu, Rb, Rt
Applications: EM, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, PEP-ELISA, PAGE, WB
NBP1-32105
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
NB100-41418
Species: Hu
Applications: IHC, IHC-P, IP, WB
NBP2-13304
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-56077
Species: Hu
Applications: IHC, IHC-P, IP, WB
NB100-56104
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
NBP1-87826
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP1-77077
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
H00010928-M02
Species: Hu, Xp
Applications: ELISA, ICC/IF, IP, WB
NBP2-94197
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-28566
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
NBP2-52947
Species: Hu, Mu, Rt
Applications: WB, IHC, KD

Publications for PGAM5 Antibody (NBP2-52947) (0)

There are no publications for PGAM5 Antibody (NBP2-52947).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PGAM5 Antibody (NBP2-52947) (0)

There are no reviews for PGAM5 Antibody (NBP2-52947). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PGAM5 Antibody (NBP2-52947) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PGAM5 Products

Research Areas for PGAM5 Antibody (NBP2-52947)

Find related products by research area.

Blogs on PGAM5

There are no specific blogs for PGAM5, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PGAM5 Antibody (CL0624) and receive a gift card or discount.

Bioinformatics

Gene Symbol PGAM5