![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Western blot analyses of periostin from soleus muscle. The graph shows quantification of periostin levels normalized to GAPDH (n = 4). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Western-Blot-NBP1-30042-img0008.jpg "Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Western blot analyses of periostin from soleus muscle. The graph shows quantification of periostin levels normalized to GAPDH (n = 4). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Ch, Bv, Ca, Eq, Fe, Av-Bi, Gt, Gp, Ha, Pm, Rb, ShSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage). |
| Immunogen | Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively-spliced exons. Accession # Q62009 |
| Specificity | Specific for the ~93 kDa periostin doublet in mouse lung extract. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes only the largest two of the three forms of periostin recognized on Western blots by the Pan periostin antibody and also shows a distinctive staining pattern by immunohistochemistry. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). |
| Predicted Species | Equine (100%), Canine (100%), Goat (100%), Hamster (100%), Sheep (100%), Bovine (100%), Guinea Pig (100%), Rabbit (100%), Finch (100%), Feline (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | POSTN |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | For IHC-P antigen retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Immunocytochemistry/Immunofluorescence was reported in scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 31430335). |
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| Theoretical MW | 93 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-P | Mouse | 12/23/2014 |
Summary
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reviewed by:
Verified Customer |
IHC | Other | 03/15/2011 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Periostin/OSF-2 Antibody (NBP1-30042)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 12/23/2014 |
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| Application: | IHC-P | |
| Species: | Mouse |
| Verified Customer 03/15/2011 |
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| Application: | IHC | |
| Species: | Other |
![Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin (red) with DAPI (blue) from soleus muscle. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-30042-img0009.jpg "Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin (red) with DAPI (blue) from soleus muscle. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.")
![Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Analysis of periostin in rabbit periosteum. Image courtesy of product review submitted by Samer Zaky.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Immunohistochemistry-NBP1-30042-img0003.jpg "Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Analysis of periostin in rabbit periosteum. Image courtesy of product review submitted by Samer Zaky.")
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Western-Blot-NBP1-30042-img0004.jpg "Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.")
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Western blot analyses of periostin. The graph shows the quantification of periostin levels normalized to GAPDH (n = 3). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Western-Blot-NBP1-30042-img0007.jpg "Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Western blot analyses of periostin. The graph shows the quantification of periostin levels normalized to GAPDH (n = 3). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin (red) with DAPI (blue) from cardiac muscle. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-30042-img0006.jpg "Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin (red) with DAPI (blue) from cardiac muscle. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.")
![Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Representative IHC images from 3 separate hearts analyzed for Periostin/OSF-2 protein expression within the infarct region of hearts from Postn^MCM/+; R26^EGFP mice versus Postn^MCM/MCM; R26^EGFP mice 2 weeks after MI. Nuclei are shown with DAPI (blue). Scale bars: 20 um. Postn lineage-traced cells (EGFP+) cells are also shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29664017/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2 Antibody - C-terminus - Azide and BSA Free-Immunohistochemistry-NBP1-30042-img0010.jpg "Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Representative IHC images from 3 separate hearts analyzed for Periostin/OSF-2 protein expression within the infarct region of hearts from Postn^MCM/+; R26^EGFP mice versus Postn^MCM/MCM; R26^EGFP mice 2 weeks after MI. Nuclei are shown with DAPI (blue). Scale bars: 20 um. Postn lineage-traced cells (EGFP+) cells are also shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29664017/) licensed under a CC-BY license.")
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Loss of Tgfbi does not alter disease progression after MI. Western blot analysis for TGFBI and periostin from isolated infarct areas of the hearts of the indicated groups of mice 7 days after MI. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0181945), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Knockout-Validated-NBP1-30042-img0005.jpg "Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Loss of Tgfbi does not alter disease progression after MI. Western blot analysis for TGFBI and periostin from isolated infarct areas of the hearts of the indicated groups of mice 7 days after MI. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0181945), licensed under a CC-BY license.")
![Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age.(A) Evaluation of cardiac function by transthoracic echocardiographic analyses. Left ventricular ejection fractions (LVEFs) are shown (n = 8–9). (B) H&E staining of cryosections from cardiac muscle. (C) Immunostaining of laminin ɑ2 (green) & periostin (red) with DAPI (blue) from cardiac muscle. (D) Western blot analyses of periostin. The graph shows the quantification of periostin levels normalized to GAPDH (n = 3). (E) qPCR analyses of Tgfb2 (TGF beta 2), Postn (periostin), & Fn1 (fibronectin) mRNA levels in cardiac muscle (n = 4). (F) qPCR analyses of Nppa (natriuretic peptide A) & Nppb (natriuretic peptide B) mRNA levels in cardiac muscle (n = 4). In qPCR analyses, data were normalized by Gapdh mRNA & expressed as fold increases from WT mice. ***P<0.001 compared with WT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31430335), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30042_rabbit-polyclonal-periostin-osf-2-antibody-c-terminus-31020241616035.jpg "Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age.(A) Evaluation of cardiac function by transthoracic echocardiographic analyses. Left ventricular ejection fractions (LVEFs) are shown (n = 8–9). (B) H&E staining of cryosections from cardiac muscle. (C) Immunostaining of laminin ɑ2 (green) & periostin (red) with DAPI (blue) from cardiac muscle. (D) Western blot analyses of periostin. The graph shows the quantification of periostin levels normalized to GAPDH (n = 3). (E) qPCR analyses of Tgfb2 (TGF beta 2), Postn (periostin), & Fn1 (fibronectin) mRNA levels in cardiac muscle (n = 4). (F) qPCR analyses of Nppa (natriuretic peptide A) & Nppb (natriuretic peptide B) mRNA levels in cardiac muscle (n = 4). In qPCR analyses, data were normalized by Gapdh mRNA & expressed as fold increases from WT mice. ***P<0.001 compared with WT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31430335), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - TGFBI & periostin are induced in the heart after injury.(A) Western blot analysis of periostin & TGFBI in isolated adult cardiomyocytes & fibroblasts. Sarcomeric alpha -actin was used as a control for cardiomyocyte purity & GAPDH was used as a loading control. (B) Quantitative real time PCR for Postn & Tgfbi from 1 week sham or MI-operated hearts. mRNA levels were normalized to 18s ribosomal RNA. *p<0.05 for both genes in MI-operated animals compared to sham animals using an unpaired Student’s T-test. n = 3 animals. (C) Western blot analysis for periostin & TGFBI in the infarcted areas isolated from hearts 24 hours, 7 & 14 days after MI surgery. GAPDH was used as a loading control. Each lane corresponds to protein from one mouse. (D & E) Quantification of TGFBI (D) & periostin (E) protein levels from the conditions shown in panel C except that 3 hearts were analyzed in total each. (F) Immunohistochemistry for the indicated markers/proteins on sham or MI-operated hearts after 1 week. Images were taken at 400x magnification. Scale = 20 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0181945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30042_rabbit-polyclonal-periostin-osf-2-antibody-c-terminus-31020241616068.jpg "Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - TGFBI & periostin are induced in the heart after injury.(A) Western blot analysis of periostin & TGFBI in isolated adult cardiomyocytes & fibroblasts. Sarcomeric alpha -actin was used as a control for cardiomyocyte purity & GAPDH was used as a loading control. (B) Quantitative real time PCR for Postn & Tgfbi from 1 week sham or MI-operated hearts. mRNA levels were normalized to 18s ribosomal RNA. *p<0.05 for both genes in MI-operated animals compared to sham animals using an unpaired Student’s T-test. n = 3 animals. (C) Western blot analysis for periostin & TGFBI in the infarcted areas isolated from hearts 24 hours, 7 & 14 days after MI surgery. GAPDH was used as a loading control. Each lane corresponds to protein from one mouse. (D & E) Quantification of TGFBI (D) & periostin (E) protein levels from the conditions shown in panel C except that 3 hearts were analyzed in total each. (F) Immunohistochemistry for the indicated markers/proteins on sham or MI-operated hearts after 1 week. Images were taken at 400x magnification. Scale = 20 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0181945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - PostnMCM allele activity in vivo.(a) Schematic representation of the Postn genetic locus with a tamoxifen-regulated MerCreMer cDNA cassette inserted into exon 1 (E1), which was crossed with Rosa26 reporter mice (R26-eGFP) containing loxP sites flanking a stop cassette upstream of eGFP to allow for Cre-dependent lineage tracing. (b) Experimental scheme whereby PostnMCM/+; R26-eGFP mice were given tamoxifen for 8 weeks before harvesting at 16 weeks. (c) Representative histological section from the heart of mice described in a & b, which show exceptionally rare labelling of interstitial cells at baseline (arrow) with 8 weeks of tamoxifen. Nuclei are stained in blue. Inset shows alpha -actin stained cardiomyocytes (red) surrounding the one eGFP-labelled interstitial cell (green) (n=4 mice). (d) Experimental scheme whereby PostnMCM/+; R26-eGFP mice were MI injured or subjected to a sham procedure, then given tamoxifen for 1 week before harvesting. (e) Western blot analysis for periostin, eGFP, Cre (MerCreMer protein) & GAPDH as a control at day 0 before injury or day 7 after MI injury with 1 week of tamoxifen (n=3 mice per condition). (f) Representative histological sections showing eGFP-labelled interstitial cells in hearts of PostnMCM/+; R26-eGFP after MI injury with 7 days of tamoxifen labelling, but not with a sham procedure (n=6 mice for MI & n=3 for sham). (g) Whole-mount fluorescent images of hearts from PostnMCM/+; R26-eGFP mice for direct eGFP fluorescence over the given time course shown. A no tamoxifen control 7 days after MI is also shown (n=3 mice per time point & condition). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27447449), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30042_rabbit-polyclonal-periostin-osf-2-antibody-c-terminus-310202416163715.jpg "Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - PostnMCM allele activity in vivo.(a) Schematic representation of the Postn genetic locus with a tamoxifen-regulated MerCreMer cDNA cassette inserted into exon 1 (E1), which was crossed with Rosa26 reporter mice (R26-eGFP) containing loxP sites flanking a stop cassette upstream of eGFP to allow for Cre-dependent lineage tracing. (b) Experimental scheme whereby PostnMCM/+; R26-eGFP mice were given tamoxifen for 8 weeks before harvesting at 16 weeks. (c) Representative histological section from the heart of mice described in a & b, which show exceptionally rare labelling of interstitial cells at baseline (arrow) with 8 weeks of tamoxifen. Nuclei are stained in blue. Inset shows alpha -actin stained cardiomyocytes (red) surrounding the one eGFP-labelled interstitial cell (green) (n=4 mice). (d) Experimental scheme whereby PostnMCM/+; R26-eGFP mice were MI injured or subjected to a sham procedure, then given tamoxifen for 1 week before harvesting. (e) Western blot analysis for periostin, eGFP, Cre (MerCreMer protein) & GAPDH as a control at day 0 before injury or day 7 after MI injury with 1 week of tamoxifen (n=3 mice per condition). (f) Representative histological sections showing eGFP-labelled interstitial cells in hearts of PostnMCM/+; R26-eGFP after MI injury with 7 days of tamoxifen labelling, but not with a sham procedure (n=6 mice for MI & n=3 for sham). (g) Whole-mount fluorescent images of hearts from PostnMCM/+; R26-eGFP mice for direct eGFP fluorescence over the given time course shown. A no tamoxifen control 7 days after MI is also shown (n=3 mice per time point & condition). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27447449), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
