![ELISA: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Indirect ELISA analysis was performed by coating the plate with recombinant NS0 cells expressed, full-length human pepsinogen A protein (97.56-1.52 nM). Coated protein was probed with Pepsinogen I antibody [HL2137] (NBP3-25594) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25594_rabbit-pepsinogen-i-mab-hl2137-azide-and-bsa-free-212024941410.jpg "ELISA: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Indirect ELISA analysis was performed by coating the plate with recombinant NS0 cells expressed, full-length human pepsinogen A protein (97.56-1.52 nM). Coated protein was probed with Pepsinogen I antibody [HL2137] (NBP3-25594) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | HL2137 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragmemt of human Pepsinogen I |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PGA3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
![Myoglobin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Myoglobin Antibody [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Pepsinogen I Antibody (NBP3-25594)Find related products by research area.
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| Gene Symbol | PGA3 |
![Western Blot: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Various tissue extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pepsinogen I antibody [HL2137] (NBP3-25594) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25594_rabbit-pepsinogen-i-mab-hl2137-azide-and-bsa-free-2120249374963.jpg "Western Blot: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Various tissue extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pepsinogen I antibody [HL2137] (NBP3-25594) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Pepsinogen I antibody [HL2137] detects Pepsinogen I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human stomach. Pepsinogen I stained by Pepsinogen I antibody [HL2137] (NBP3-25594) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25594_rabbit-pepsinogen-i-mab-hl2137-azide-and-bsa-free-2120249413397.jpg "Immunohistochemistry-Paraffin: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Pepsinogen I antibody [HL2137] detects Pepsinogen I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human stomach. Pepsinogen I stained by Pepsinogen I antibody [HL2137] (NBP3-25594) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Pepsinogen I antibody [HL2137] detects Pepsinogen I protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Pepsinogen I stained by Pepsinogen I antibody [HL2137] (NBP3-25594) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25594_rabbit-pepsinogen-i-mab-hl2137-azide-and-bsa-free-2120249413399.jpg "Immunocytochemistry/Immunofluorescence: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Pepsinogen I antibody [HL2137] detects Pepsinogen I protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Pepsinogen I stained by Pepsinogen I antibody [HL2137] (NBP3-25594) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pepsinogen I antibody [HL2137] (NBP3-25594) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25594_rabbit-pepsinogen-i-mab-hl2137-azide-and-bsa-free-2120249425440.jpg "Western Blot: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pepsinogen I antibody [HL2137] (NBP3-25594) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
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![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")